PMID: 9179597Jun 1, 1997Paper

Identification of the delayed rectifier potassium channel, Kv1.6, in cultured astrocytes

Glia
S L SmartB L Tempel

Abstract

Astrocytes are an abundant glial cell type of the central nervous system that appear to play a role in regulating extracellular potassium concentrations in brain, thereby contributing to the maintenance of normal neuronal activity. Voltage-gated potassium conductances, shown to be present in astrocytes, may be involved in this and other astrocytic functions. Toward defining the role of voltage-gated potassium channels in astrocytes, total RNA prepared from cultured mouse cortical astrocytes was screened, using a reverse transcriptase-polymerase chain reaction (RT-PCR) approach, for the expression of several members of the Shaker-like potassium channel subfamily (Kv1.1-Kv1.6). A relatively high level of Kv1.6 transcript was identified by RT-PCR and then confirmed and quantitated by ribonuclease protection assays using a Kv1.6-specific riboprobe. Immunocytochemical staining showed double-labeling of glial fibrillary acidic protein-positive cells with antibody specific for the Kv1.6 channel. The Kv1.6 protein expression was variable among the individual astrocytes. Outward voltage-gated currents were studied in astrocytes in primary culture using the Nystatin-perforated patch voltage clamp technique. Outward potassium currents wer...Continue Reading

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Citations

Jan 17, 2002·The Journal of Comparative Neurology·Lori EdwardsMichael G Fehlings
Sep 9, 2008·Pflügers Archiv : European journal of physiology·Zoltán Rusznák, Géza Szucs
Aug 12, 2006·Journal of Neurocytology·Daniel Reyes-HaroJesús García-Colunga
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Sep 21, 2004·Glia·Andrzej BaciaBetty Soliven
Oct 25, 2003·Biochemical and Biophysical Research Communications·G GuihardD Escande
Dec 3, 2014·Autoimmunity Reviews·Peter Jukkola, Chen Gu
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Dec 12, 2002·American Journal of Physiology. Cell Physiology·Betty SolivenTamaki Iwase
Feb 5, 2010·American Journal of Physiology. Cell Physiology·Yi HanMan-Wen Jin
Jun 8, 2021·Frontiers in Cellular Neuroscience·Jessica McNeillNatalina Salmaso
Nov 5, 2021·Pflügers Archiv : European journal of physiology·Francesco FormaggioStefano Ferroni

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