Identification of the differentially expressed protein biomarkers in rat blood plasma in response to gamma irradiation

International Journal of Radiation Biology
Jia-Li SunQing-Jie Liu

Abstract

Purpose: Simple, rapid and high-throughput dose assessment is critical for clinical diagnosis, treatment and emergency intervention in a large-scale radiological accident. The goal of this study is to screen and identify new ionizing radiation-responsive protein biomarkers in rat plasma.Materials and methods: Sprague-Dawley rats were exposed to single doses of 0, 1, 3, 5 Gy of Cobalt-60 γ-rays total body irradiation at a dose rate of 1 Gy/min. The tandem mass tag labeling (TMT) combined with liquid chromatography mass spectrometry (LC-MS/MS) approach was used to screen the differentially expressed proteins in rat plasma collected at 1, 3, 5 and 7 days post-irradiation. Bioinformatics analysis was conducted to explore the biological functions of these proteins. The expression levels of candidate radiation-sensitive protein biomarkers were confirmed using enzyme-linked immune-sorbent assay (ELISA).Results: A total of 503 differentially expressed proteins were identified. Most of these proteins were implicated in immune response, phagocytosis and signal transduction following ionizing radiation. Five up-regulated proteins including alpha-2-macroglobulin (A2m), chromogranin-A (CHGA), glutathione pertidase 3 (GPX3), clusterin (Clu) ...Continue Reading

References

Aug 15, 2002·The Journal of Biological Chemistry·Patrice VachetteBenedetto Salvato
Apr 26, 2007·International Journal of Radiation Biology·Emma AssemandMircea Alexandru Mateescu
Mar 18, 2009·Biochimica Et Biophysica Acta·Regina Brigelius-Flohé, Anna Kipp
Apr 15, 2009·International Journal of Radiation Biology·Kanokporn Noy RithidechSanford R Simon
Nov 12, 2010·International Journal of Radiation Biology·Sylwia KabacikChristophe Badie
Dec 25, 2010·Radiation Protection Dosimetry·S G Andreev, Y A Eidelman
Mar 31, 2012·The Journal of Biological Chemistry·Hui WangYan P Yu
Apr 18, 2012·The Journal of Experimental Medicine·Olivier HeraultGuy Sauvageau
Oct 23, 2012·Journal of Cellular Physiology·Ahmed A RehmanFahim H Khan
Nov 14, 2012·Molecular & Cellular Proteomics : MCP·Thibault ChazeOlivier Guipaud
Jul 28, 2013·Radiation Research·C BadieM Abend
Aug 14, 2013·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Daiki IkedaHarumoto Yamada
Mar 14, 2014·The Lancet. Diabetes & Endocrinology·Jens P GoetzeJens F Rehfeld
Mar 29, 2014·OncoTargets and Therapy·Tomas Koltai
May 3, 2014·Oxidative Medicine and Cellular Longevity·Mona M MohamedWael El-Rifai
Jun 14, 2014·Radiation Protection Dosimetry·Natalia I OssetrovaWilliam F Blakely
Aug 3, 2014·International Journal of Radiation Oncology, Biology, Physics·Jose G BazanDavid E Cooper
Oct 30, 2015·Radiation and Environmental Biophysics·Li-hua XieHai-qian Zhang
Jan 9, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Alihossein SaberiManoochehr Makvandi
Nov 10, 2017·Mass Spectrometry Reviews·Robert MoulderRiitta Lahesmaa
Aug 4, 2018·Endokrynologia Polska·Zoltan HeroldAniko Somogyi
May 28, 2019·Journal of Cellular Physiology·Qiang MaXiaolan Guo
Sep 7, 2019·Cell Biochemistry and Biophysics·Laura CalvertAnthony Chan

❮ Previous
Next ❯

Citations

Nov 5, 2020·Biomedicines·Elena ObradorAlegría Montoro
Nov 3, 2020·Drug Discovery Today·Sandeep Kumar ShuklaM H Yashavarddhan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
X ChenW H Jiang
Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte
Nour EissaJean-Eric Ghia
© 2021 Meta ULC. All rights reserved