PMID: 8952000Nov 14, 1996Paper

Identification of the dipteran Leu-callatostatin peptide family: the pattern of precursor processing revealed by isolation studies in Calliphora vomitoria

Regulatory Peptides
Hanne DuveA Thorpe

Abstract

Information from the Leu-callatostatin gene sequences of the blowflies Calliphora vomitoria and Lucilia cuprina was used to develop antisera specific for the variable post-tyrosyl amino-acid residues Ser, Ala and Asn of the common Leu-callatostatin C-terminal pentapeptide sequence -YXFGL-NH2. Radioimmunoassays based on these antisera were used to purify peptides from an extract of 40000 blowfly heads. Five neuropeptides of the Leu-callatostatin family were identified. Three have a seryl residue in the post-tyrosyl position. Two of these are octapeptides that differ only at the N-terminal residue; NRPYSFGL-NH2 and ARPYSFGL-NH2, whilst the third is the heptapeptide derived by N-terminal trimming; RPYSFGL-NH2. Two octapeptides in which X is Ala and Asn were also identified; VERYAFGL-NH2 and LPVYNFGL-NH2. The latter peptide is derived by processing at the internal dibasic site of a putative heneicosapeptide encoded by the DNA. These findings stress the necessity to have putative structures verified at the peptide level. Potent, reversible inhibitory effects on the spontaneous contractile activity of the blowfly rectum were recorded for ARPYSFGL-NH2 (monophasic dose-response curve with an IC50 = 10 fM) and for LPVYNFGL-NH2 (biphasic...Continue Reading

References

Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G E PrattR Feyereisen
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A P WoodheadS S Tobe
Sep 29, 1989·Biochemical and Biophysical Research Communications·G E PrattR Feyereisen
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·K ReichwaldR Feyereisen
Mar 1, 1994·Insect Biochemistry and Molecular Biology·A P WoodheadS S Tobe
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B C DonlyW G Bendena
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H DuveA Thorpe

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Citations

Dec 16, 2004·Archives of Insect Biochemistry and Physiology·Marlene DaveyPeter East
Mar 15, 2006·Cell and Tissue Research·Petra SkiebeHans-Joachim Pflüger
Jan 1, 1997·Peptides·H DuveA Thorpe
Nov 6, 2001·Molecular and Cellular Endocrinology·M Meyering-VosF Sehnal
Jun 26, 1999·The European Journal of Neuroscience·S KreisslW Rathmayer
Feb 17, 2000·Annals of the New York Academy of Sciences·W G BendenaS S Tobe
Jul 6, 2000·Insect Biochemistry and Molecular Biology·L I GilbertR M Roe
Oct 30, 2015·Archives of Insect Biochemistry and Physiology·Franziska WendeKlaus H Hoffmann
Oct 19, 2010·Peptides·Francisco Martínez-PérezStephen S Tobe
Jun 25, 2009·Peptides·Francisco Martínez-PérezStephen S Tobe
Sep 6, 2002·Journal of Peptide Science : an Official Publication of the European Peptide Society·I N AlievaN M Godjaev
Jan 25, 2000·Archives of Insect Biochemistry and Physiology·J A Veenstra

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