PMID: 9443972Mar 14, 1998Paper

Identification of the DNA sequence that interacts with the gut-enriched Krüppel-like factor

Nucleic Acids Research
J M Shields, V W Yang

Abstract

The gut-enriched Krüppel-like factor (GKLF) is a recently identified eukaryotic transcription factor that contains three C2H2zinc fingers. The amino acid sequence of the zinc finger portion of GKLF is closely related to several Krüppel proteins, including the lung Krüppel-like factor (LKLF), the erythroid Krüppel-like factor (EKLF) and the basic transcription element binding protein 2 (BTEB2). The DNA sequence to which GKLF binds has not been definitively established. In the present study we determined the DNA binding sequence of GKLF using highly purified recombinant GKLF in a target detection assay of an oligonucleotide library consisting of random sequences. Upon repeated rounds of selection and subsequent characterization of the selected sequences by base-specific mutagenesis a DNA with the sequence 5'-G/AG/AGGC/TGC/T-3' was found to contain the minimal essential binding site for GKLF. This sequence is present in the promoters of two previously characterized genes: the CACCC element of the beta-globin gene, which interacts with EKLF, and the basic transcription element (BTE) of the CYP1A1 gene, which interacts with Sp1 and several Sp1-like transcription factors. Moreover, the selected GKLF binding sequence was capable of me...Continue Reading

References

Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M Berg
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B A ChristyD Nathans
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H R Pelham, D D Brown
Apr 11, 1993·Nucleic Acids Research·K SogawaY Fujii-Kuriyama
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·A C JamiesonS H Kim

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Citations

Aug 24, 1999·European Journal of Immunology·S PorcelliniF Grassi
Sep 6, 2000·Journal of Cellular Biochemistry·H A FeisterJ P Bidwell
Apr 2, 2008·Cell Stress & Chaperones·Ying LiuXianzhong Xiao
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·D T DangV W Yang
Dec 24, 2005·Nature Reviews. Cancer·Benjamin D Rowland, Daniel S Peeper
Nov 25, 2003·The Journal of Biological Chemistry·Silvia A PiccinniOlivier Lefebvre
Nov 25, 2003·The Journal of Biological Chemistry·Hong S Yoon, Vincent W Yang
Aug 23, 2003·DNA and Cell Biology·Arthur G BallietDan A Liebermann
Aug 24, 1999·Nucleic Acids Research·S Philipsen, G Suske
Jun 21, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jie AnNanping Weng
Feb 7, 2007·Molecular and Cellular Biology·Alister P W FunnellMerlin Crossley
Oct 21, 2010·Physiological Reviews·Beth B McConnell, Vincent W Yang
Mar 4, 2008·Molecular Cancer Research : MCR·Shuang LiuYan Dong
Jul 22, 2006·American Journal of Respiratory Cell and Molecular Biology·Biao HuSem H Phan
Aug 11, 2007·BMC Medical Genetics·Ruth Gutiérrez-AguilarBernadette Neve
Jun 1, 2010·Human Genomics·Shivalingappa K Swamynathan
Feb 17, 2010·Genome Biology·Stoyan GeorgievUwe Ohler
May 9, 2014·The Journal of Clinical Investigation·Kaori HayashiHiroshi Itoh
Feb 13, 2014·Nucleic Acids Research·Yiwei LiuXiaodong Cheng
Jul 13, 2011·Molecular and Cellular Biology·Glen A BjerkeDavid Wotton
Feb 4, 2011·Cellular and Molecular Life Sciences : CMLS·Anja SchuetzDirk Carstanjen
May 28, 2008·Current Colorectal Cancer Reports·Amr M Ghaleb, Vincent W Yang
Feb 5, 2013·Biochemical and Biophysical Research Communications·Benjamin KoopmanschRosita Winkler
Jun 26, 2001·Journal of Cellular Physiology·A R BlackJ Azizkhan-Clifford
Nov 14, 2014·The FEBS Journal·Kate Beishline, Jane Azizkhan-Clifford
Dec 31, 2011·Experimental Cell Research·Ikumi MichikamiMakoto Abe

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