Identification of the dopamine transporter SLC6A3 as a biomarker for patients with renal cell carcinoma

Molecular Cancer
Sarah SchrödterJörg Ellinger

Abstract

Clear cell renal cell carcinoma (ccRCC) is among the most common human malignancies. In order to provide better understanding of the molecular biology of ccRCC and to identify potential diagnostic/prognostic biomarker and therapeutic targets, we utilized a microarray to profile mRNA expression of corresponding normal and malignant renal tissues. Real-time PCR, Western Blot and immunohistochemistry were applied to study the expression of candidate biomarkers. ccRCC cell lines were treated with sertraline to inhibit the dopamine transporter SLC6A3. Differential expression of fourteen mRNAs, yet not studied in ccRCC in depth, was confirmed using qPCR (upregulation: SLC6A3, NPTX2, TNFAIP6, NDUFA4L2, ENPP3, FABP6, SPINK13; downregulation: FXYD4, SLC12A1, KNG1, NPHS2, SLC13A3, GCGR, PLG). Up-/downregulation was also confirmed for FXYD4, KNG1, NPTX2 and SLC12A1 by Western Blot on the protein level. In contrast to the mRNA expression, protein expression of the dopamine transporter SLC6A3 was lower in ccRCC compared to normal renal tissue. Immunohistochemistry indicated that this decrease was due to higher concentrations of SLC6A3 in the proximal tubules. Immunohistochemical analyses further demonstrated that high SLC6A3 expression in c...Continue Reading

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Citations

Jul 8, 2019·International Journal of Urology : Official Journal of the Japanese Urological Association·Johannes SteinJörg Ellinger
May 11, 2020·Cancer Metastasis Reviews·Ludger Johannes, Anne Billet
Sep 11, 2018·Cancer Biomarkers : Section a of Disease Markers·Jianmin LiuYan Wang
Sep 26, 2017·Cancer Treatment and Research Communications·Adriana ZingoneBríd M Ryan
Mar 24, 2021·Molecular Carcinogenesis·Felix von HagenJörg Ellinger
Apr 23, 2021·Pathology, Research and Practice·Sinan LiuRongrong Jing

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Methods Mentioned

BETA
PCR
ELISA
electrophoresis
Protein
enzyme linked immunosorbent assay

Software Mentioned

SPSS Statistics
R
Biogazelle
NextBio tool
Tissue Studie
SPSS
Qbase
gplot
Ensembl

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