Identification of the down-regulated genes in a mat1-2-deleted strain of Gibberella zeae, using cDNA subtraction and microarray analysis

Fungal Genetics and Biology : FG & B
Seung-Ho LeeSung-Hwan Yun

Abstract

Gibberella zeae (anamorph: Fusarium graminearum), a self-fertile ascomycete, is an important pathogen of cereal crops. Here, we have focused on the genes specifically controlled by the mating type (MAT) locus, a master regulator of sexual developmental process in G. zeae. To identify these genes, we employed suppression subtractive hybridization between a G. zeae wild-type strain Z03643 and the isogenic self-sterile mat1-2 strain T43deltaM2-2. Both reverse Northern and cDNA microarray analyses using 291 subtractive unigenes confirmed that 58.8% (171 genes) were significantly down-regulated in T43deltaM2-2. Among these, 98 could be either manually or automatically annotated based on known functions of their possible homologs. Northern blot analysis revealed that all of the genes examined were differentially regulated by MAT1-2 during sexual development. This study is the first report on the set of genes that are transcriptionally altered by the deletion of MAT1-2 during sexual reproduction in G. zeae.

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Citations

Jan 18, 2011·Fungal Genetics and Biology : FG & B·Hokyoung SonYin-Won Lee
Sep 15, 2010·Biochemical and Biophysical Research Communications·Jungkwan LeeYin-Won Lee
Jun 24, 2008·Fungal Genetics and Biology : FG & B·Hee-Kyoung KimSung-Hwan Yun
Aug 28, 2007·International Journal of Food Microbiology·László HornokJohn F Leslie
Feb 17, 2007·Molecular Microbiology·You-Kyoung HanYin-Won Lee
Jul 5, 2011·Molecular Plant Pathology·Wilfried JonkersCaroline B Michielse
Feb 6, 2008·Fungal Genetics and Biology : FG & B·Raphaël CarapitoVincent Phalip
May 29, 2020·Phytopathology·Elizabeth K BrauerLinda J Harris
Dec 23, 2006·Microbiology·Andrew Breakspear, Michelle Momany
Oct 21, 2016·Microbiology Spectrum·Richard J Bennett, B Gillian Turgeon

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