Identification of the Eph receptor pathway as a novel target for eicosapentaenoic acid (EPA) modification of gene expression in human colon adenocarcinoma cells (HT-29).

Nutrition & Metabolism
Joanne F DolemanElizabeth K Lund

Abstract

The health benefits of polyunsaturated fatty acids (PUFAs), particularly those of the n-3 series are well documented. The mechanisms by which these effects are mediated are not fully clarified. We used microarrays to assess the effects on gene expression in HT29 colon adenocarcinoma cells of exposure to the n-3 fatty acid eicosapentaenoic acid (EPA). HT29 cells were cultured with EPA (150 muM) for up to 24 hr prior to harvesting and isolation of RNA. Microarray results were analyzed within the statistical package 'R', and GeneGo MetaCore was used to identify key pathways of altered gene expression. EphB4, Vav2 and EphA1 gene expression were identified as significantly altered by EPA treatment. Statistically significant changes in gene expression after HT29 exposure to EPA were confirmed in a second experiment by real-time RT-PCR (TaqMan), This experiment also compared the effects of exposure to EPA to arachadonic acid (AA, n-6). Corresponding changes in protein expression were also assessed by Western blotting. Eph receptor mediated signaling is an entirely novel signaling pathway through which EPA may promote a wide range of health benefits, in particular in relation to reduction of colorectal cancer progression.

References

Jan 1, 1995·Annals of Human Genetics·E P HenskeD J Kwiatkowski
Feb 8, 2000·The International Journal of Biochemistry & Cell Biology·M Nakamoto
Aug 10, 2000·The Journal of Experimental Medicine·D D BilladeauP J Leibson
Jul 13, 2001·The Journal of Cell Biology·P A Marignani, C L Carpenter
Mar 29, 2002·Cancer·Wenbiao LiuLee M Ellis
May 30, 2002·Biological Chemistry·Dean G TangJames P Kehrer
Mar 19, 2004·Pathology Oncology Research : POR·Qinghua WuJahn M Nesland
Mar 20, 2004·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Catherine E RoynetteClaude Pichard
Jun 24, 2005·Nature·Eduard BatlleHans Clevers
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Jan 7, 2006·Cancer Research·Hans Clevers, Eduard Batlle
Feb 28, 2006·Gynecologic Oncology·Qinghua WuJahn M Nesland
May 25, 2006·Heredity·T Svingen, K F Tonissen
Jun 27, 2007·Alimentary Pharmacology & Therapeutics·I T Johnson, E K Lund
Sep 8, 2007·American Journal of Epidemiology·Anouk GeelenEllen Kampman
Feb 5, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Kevin McHaleCharles V Clevenger
Feb 19, 2008·Epigenetics : Official Journal of the DNA Methylation Society·Qinghua WuZhenhe Suo
Mar 6, 2009·The Journal of Nutrition, Health & Aging·A D DangourR Uauy
Jul 24, 2012·International Journal of Cancer. Journal International Du Cancer·Claudia AgnoliVittorio Krogh

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Citations

Dec 31, 2010·World Journal of Gastrointestinal Oncology·Haruhiko SugimuraXiao-Jun Zhou
Mar 10, 2011·The Proceedings of the Nutrition Society·Elizabeth K LundIan T Johnson

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Methods Mentioned

BETA
biopsy
chips
Assay
nucleotide exchange

Software Mentioned

GeneGo [UNK]
MetaCore
GQuantile
R
GeneGo MetaCore
Limma
Axon GenePix
Minitab Package
ArrayExpress
GeneGo

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