Abstract
GTP-binding protein/transglutaminases (tissue transglutaminases or TGases) have been implicated in a variety of cellular processes including retinoic acid (RA)-induced apoptosis. Recently, we have shown that RA activates TGases as reflected by stimulated GTP binding, increased membrane association, and stimulated phosphoinositide lipid turnover. This prompted us to search for cellular proteins that bind TGases in a RA-stimulated manner. In this report, we show that the eukaryotic initiation factor (eIF-5A), a protein that is essential for cell viability, perhaps through effects on protein synthesis and/or RNA export, associates with the TGase in vivo. The interaction between eIF-5A and TGase is specific for the GDP-bound form of the TGase and is not detected when the TGase is pre-loaded with GTP gamma S. The TGase-eIF-5A interaction also is promoted by Ca2+, Mg2+, and RA treatment of HeLa cells. In the presence of retinoic acid, millimolar levels of Ca2+ are no longer required for the TGase-eIF-5A interaction. Nocodazole treatment, which blocks the cell cycle at mitosis (M phase), strongly inhibits the interaction between eIF-5A and cytosolic TGase. The interaction between TGase and eIF-5A and its sensitivity to the nucleotide-...Continue Reading
References
Oct 1, 1992·The Journal of Cell Biology·V GentileP J Davies
Mar 29, 1991·Biochemical and Biophysical Research Communications·D CaiL M De Luca
Dec 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C S GreenbergR H Rice
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A L KungR T Schimke
Aug 15, 1989·Biochemical and Biophysical Research Communications·K N LeeM K Patterson
Dec 8, 1972·Annals of the New York Academy of Sciences·S I Chung
Aug 15, 1970·Nature·U K Laemmli
Jan 1, 1980·Annual Review of Biochemistry·J E Folk
Dec 5, 1995·Biochemistry·U S SinghR A Cerione
Nov 10, 1995·The Journal of Biological Chemistry·K C HwangM J Im
Aug 14, 1995·FEBS Letters·S MianM Griffin
Feb 1, 1995·The Biochemical Journal·S BeninatiA Abbruzzese
Jun 10, 1994·Science·H NakaokaR M Graham
Oct 1, 1994·Molecular and Cellular Biology·G MelinoM Piacentini
Jan 1, 1995·Journal of Cellular Biochemistry. Supplement·L FésüsI Uray
Jan 5, 1996·Biochemical and Biophysical Research Communications·B M Fraij
Mar 8, 1996·Science·R MittalR A Cerione
Mar 29, 1996·Science·D BevecJ Hauber
Jul 12, 1996·The Journal of Biological Chemistry·J F FengM J Im
Aug 23, 1996·Cell·E J Brown, S L Schreiber
Nov 1, 1996·The Journal of Biological Chemistry·U S Singh, R A Cerione
Dec 6, 1996·The Journal of Biological Chemistry·T S LaiC S Greenberg
Jun 1, 1996·Amino Acids·M H ParkE C Wolff
Citations
Mar 1, 2002·Electrophoresis·Kana TakeuchiKiwamu Okita
Jun 20, 2002·Journal of Immunological Methods·Alessandra AmendolaZsuzsa Szondy
Feb 13, 2001·Neuroscience·J TucholskiG V Johnson
Jan 5, 2000·Insect Molecular Biology·M M van OersA A Thomas
Jun 18, 2002·Journal of Neurochemistry·Janusz Tucholski, Gail V W Johnson
May 15, 2001·The EMBO Journal·U S SinghK M Baker
May 22, 2007·Plant Physiology·Haizhong FengJianru Zuo
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Shenping LiuJon Clardy
Nov 5, 2011·The FEBS Journal·Ting-Fang KuoSoichi Kojima
Oct 8, 2004·Journal of Biochemistry and Molecular Biology·Soo-Mi KweonSkwon-Soo Ha
Oct 16, 2012·Journal of Ethnopharmacology·Dong-Feng WeiYing-Cun Bao
Dec 3, 2013·Journal of Proteomics·Sara SilenoOrnella Massa
May 7, 2015·Cellular and Molecular Life Sciences : CMLS·Kajal KanchanLászló Fésüs
Jun 3, 2016·Cell Death & Disease·H TatsukawaS Kojima
Jun 9, 2016·Amino Acids·Thung-S LaiCharles S Greenberg
Nov 6, 1998·FEBS Letters·S BeninatiA Abbruzzese
Oct 11, 2005·Journal of Cellular Biochemistry·David Li-En Jao, Kuang Yu Chen
Sep 30, 2006·Blood·Stefan BalabanovTim H Brümmendorf
Apr 17, 2002·The Journal of Biological Chemistry·Jason E BoehmRichard A Cerione
Nov 4, 2000·The Journal of Biological Chemistry·A Xu, K Y Chen
Oct 29, 2002·The Journal of Biological Chemistry·Ugra S SinghKenneth M Baker
Dec 11, 2007·The FEBS Journal·Veridiana S P CanoMyung Hee Park