Identification of the feline humoral immune response to Bartonella henselae infection by protein microarray.

PloS One
Adam VigilPhilip L Felgner

Abstract

Bartonella henselae is the zoonotic agent of cat scratch disease and causes potentially fatal infections in immunocompromised patients. Understanding the complex interactions between the host's immune system and bacterial pathogens is central to the field of infectious diseases and to the development of effective diagnostics and vaccines. We report the development of a microarray comprised of proteins expressed from 96% (1433/1493) of the predicted ORFs encoded by the genome of the zoonotic pathogen Bartonella henselae. The array was probed with a collection of 62 uninfected, 62 infected, and 8 "specific-pathogen free" naïve cat sera, to profile the antibody repertoire elicited during natural Bartonella henselae infection. We found that 7.3% of the B. henselae proteins on the microarray were seroreactive and that seroreactivity was not evenly distributed between predicted protein function or subcellular localization. Membrane proteins were significantly most likely to be seroreactive, although only 23% of the membrane proteins were reactive. Conversely, we found that proteins involved in amino acid transport and metabolism were significantly underrepresented and did not contain any seroreactive antigens. Of all seroreactive ant...Continue Reading

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Citations

Jul 16, 2014·Zebrafish·Amorce LimaBurt Anderson
Oct 22, 2013·Journal of Proteomics·Raúl Manzano-RománManuel Fuentes
Aug 2, 2011·Genome Medicine·Malgorzata KowalczewskaDidier Raoult
Aug 6, 2011·Molecular & Cellular Proteomics : MCP·Adam VigilPhilip L Felgner
Jan 11, 2012·Clinical Microbiology Reviews·Alexander Harms, Christoph Dehio
Oct 3, 2015·Vaccine·D Huw DaviesDenise L Doolan
Oct 31, 2014·Journal of Proteome Research·Carolina Lessa-AquinoPhilip L Felgner
Apr 24, 2012·Cellular Microbiology·Simone C Eicher, Christoph Dehio
May 20, 2014·Letters in Applied Microbiology·F FerraraD Sblattero
Aug 4, 2015·Current Opinion in Infectious Diseases·Li Liang, Philip L Felgner
Oct 12, 2010·Bioinformatics·Christophe N MagnanPierre Baldi
Jul 18, 2015·Molecular & Cellular Proteomics : MCP·Alfonso Olaya-AbrilManuel J Rodríguez-Ortega
May 12, 2017·Clinical Microbiology Reviews·Udoka OkaroBurt Anderson

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Methods Mentioned

BETA
in vitro transcription
protein array
electrophoresis
PCR
chip
ELISA

Software Mentioned

ImageJ
Photoshop
MIAME
pSORTb
âe1071â
Bioconductor suite
R
TMHMM
vsn
QuantArray

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