Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae

Scientific Reports
Sílvio B SantosJoana Azeredo

Abstract

Bacteriophage endolysins present enormous biotechnological potentials and have been successfully used to control and detect bacterial pathogens. Endolysins targeting Gram-positive bacteria are modular, displaying a cell binding (CBD) and an enzymatically active domain. The CBD of phage endolysins are recognized by their high specificity and host affinity, characteristics that make them promising diagnostic tools. No CBD able to bind Paenibacillus larvae has been identified so far. P. larvae is a Gram-positive spore forming bacteria that causes the American Foulbrood. This highly contagious infection leads to honeybee larvae sepsis and death, resulting in an adverse impact on pollination and on the beekeeping industry. In this work, the first CBD targeting P. larvae was identified and its core binding sequence was investigated. Moreover, it was shown that the domain is highly specific, targeting exclusively P. larvae cells from all ERIC genotypes. The identification of such a domain represents a step forward for the development of effective methods to detect and control this pathogen.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 5, 1997·Applied and Environmental Microbiology·I G Wilson
May 23, 2002·Biochemistry·A Keith DunkerZoran Obradović
Mar 22, 2006·Expert Opinion on Investigational Drugs·Glenn S TillotsonJoseph M Blondeau
Sep 26, 2006·Journal of Pharmaceutical Sciences·Wei WangSandeep Nema
Nov 7, 2006·Applied and Environmental Microbiology·Peter Sass, Gabriele Bierbaum
Dec 13, 2006·Nature Biotechnology·Vincent A FischettiRaymond Schuch
Apr 25, 2007·Nucleic Acids Research·Warren A Kibbe
Aug 5, 2008·Biosensors & Bioelectronics·Peter Durand SkottrupAnnemarie Fejer Justesen
Nov 17, 2009·Journal of Invertebrate Pathology·Elke Genersch
Jun 23, 2012·The Analyst·Amit SinghStephane Evoy
Oct 4, 2012·Future Microbiology·Mathias SchmelcherMartin J Loessner
Oct 26, 2012·TheScientificWorldJournal·Noori Al-WailiMohammad Javed Ansari
Feb 15, 2013·Journal of Virology·Hugo OliveiraLeon D Kluskens
Aug 31, 2013·PloS One·Svjetlana VojvodicKirk E Anderson
Sep 13, 2013·Journal of the Royal Society, Interface·Samik DattaMatt J Keeling
Oct 31, 2013·Methods in Molecular Biology·Punto Bawono, Jaap Heringa
Jan 24, 2014·Bioinformatics·Philip JonesSarah Hunter
Feb 18, 2014·Bacteriophage·Mathias Schmelcher, Martin J Loessner
Sep 23, 2014·Applied and Environmental Microbiology·Vanessa Corby-HarrisKirk E Anderson
Dec 10, 2014·Bioinformatics·Ilan SamishRafael J Najmanovich
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Jun 7, 2015·Applied and Environmental Microbiology·Hannes BeimsMichael Steinert
Jun 19, 2015·FEMS Microbiology Letters·Jana UgorcakovaGabriela Bukovska
Jun 20, 2015·Genome Announcements·Susan CarsonEric S Miller
Jan 9, 2016·Genome Announcements·J AbrahamE S Miller
Oct 16, 2016·Bacteriophage·Casey StamereilersPhilippos K Tsourkas
Dec 3, 2016·Nucleic Acids Research·UNKNOWN NCBI Resource Coordinators
Dec 21, 2017·Journal of Molecular Biology·Lukas ZimmermannVikram Alva

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
fluorescence microscopy
Protein Assay

Software Mentioned

Praline
InterProScan
BLASTp
HHpred
Phyre2
iCn3D Structure Viewer
PlyPl23
Motif
OligoCalc

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.