PMID: 8960115Aug 1, 1996Paper

Identification of the Fy6 epitope recognized by two monoclonal antibodies in the N-terminal extracellular portion of the Duffy antigen receptor for chemokines

Molecular Immunology
K WasniowskaE Lisowska

Abstract

The epitope Fy6 recognized by two monoclonal antibodies (i3A and BG6), which inhibit binding of chemokines to the Duffy antigen, was characterized by means of peptides synthesized on pins (Epitope Scanning Kit) and deletion mutagenesis. Both antibodies showed very similar specificities. They recognized a linear epitope, the essential portion of which was the heptapeptide Gln-Leu-Asp-Phe-Glu-Asp-Val comprising amino acid residues 21-27, located between two glycosylation sites of the Duffy protein. All the amino acid residues of the epitope, except Glu, were essential for antibody binding, since they could not be replaced by any other amino acid residues or by only one or two. The Glu residue could be replaced by most other amino acid residues, and its replacement by 10 amino acid residues gave a distinct increase in the antibody binding. The results were in full agreement with the finding that the mutant of the Duffy antigen, lacking amino acid residues 23-25 (-Asp-Phe-Glu-), did not bind the i3A antibody, but bound the anti-Fy3 monoclonal antibody similarly to the wild type of the Duffy antigen. The apparent affinity constants of both anti-Fy6 antibodies were determined by surface plasmon resonance, using immunopurified Duffy p...Continue Reading

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Citations

Mar 30, 2000·Transfusion·K YazdanbakhshM E Reid
Jun 30, 2000·Biochemical and Biophysical Research Communications·K WaśniowskaE Lisowska
Nov 23, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·William P SheffieldGregory A Denomme
Jul 4, 2007·Hematology·Dante M Langhi, José Orlando Bordin
Jul 19, 2005·Biochemical and Biophysical Research Communications·Steven L ParkerFloyd Sallee
Mar 27, 2007·Biochemical and Biophysical Research Communications·Marcin CzerwinskiKazimiera Wasniowska
Jul 19, 2005·Molecular and Biochemical Parasitology·Christophe TournamilleYves Colin
Feb 19, 2000·American Journal of Hematology·K YazdanbakhshM E Reid
Jun 12, 1998·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J P Cartron
Dec 8, 2000·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·C Tournamille
May 3, 2000·Seminars in Hematology·A O Pogo, A Chaudhuri
Jul 27, 2010·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·D SmolarekA G de Brevern

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