Identification of the gene encoding hydroxyacid-oxoacid transhydrogenase, an enzyme that metabolizes 4-hydroxybutyrate

FEBS Letters
Tamas KardonEmile Van Schaftingen

Abstract

To identify the sequence of hydroxyacid-oxoacid transhydrogenase (HOT), responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues, we have purified this enzyme from rat liver and obtained partial sequences of proteins coeluting with the enzymatic activity in the last purification step. One of the identified proteins was 'iron-dependent alcohol dehydrogenase', an enzyme encoded by a gene present on human chromosome 8q 13.1 and distantly related to bacterial 4-hydroxybutyrate dehydrogenases. The identification of this protein as HOT was confirmed by showing that overexpression of the mouse homologue in HEK cells resulted in the appearance of an enzyme catalyzing the alpha-ketoglutarate-dependent oxidation of 4-hydroxybutyrate to succinate semialdehyde.

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Citations

Jun 9, 2009·PloS One·Jingjing GuEmmeline W Hill
Jan 9, 2013·Journal of Inherited Metabolic Disease·Emile Van SchaftingenCarole L Linster
Nov 21, 2008·Journal of Inherited Metabolic Disease·E Van SchaftingenM Veiga-da-Cunha
Feb 19, 2011·Chemico-biological Interactions·Aída Hernández-TobíasHéctor Riveros-Rosas
Dec 13, 2006·Biochemical and Biophysical Research Communications·Younes AchouriEmile Van Schaftingen
Jul 23, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Vladimir A NaumovVadim M Govorun
Dec 3, 2014·Chemico-biological Interactions·Hans JörnvallLinus J Östberg
May 26, 2017·International Journal of Molecular Sciences·Hye-Kyung Na, Ja Young Lee
Oct 6, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yehuda Shabtai, Abraham Fainsod
Jan 2, 2019·Molecular & Cellular Proteomics : MCP·Ryan DosselliBoris Baer
Aug 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Wen ZhangPing Xu
Mar 15, 2019·Molecular & Cellular Proteomics : MCP·Ryan DosselliBoris Baer
Jun 29, 2021·Open Life Sciences·Qi ChenYaojun Peng

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