Identification of the gene encoding scHelI, a DNA helicase from Saccharomyces cerevisiae

Yeast
D W Bean, S W Matson

Abstract

The gene encoding scHelI, a previously characterized DNA helicase from Saccharomyces cerevisiae, has been identified as YER176w, an open reading frame on chromosome V. The gene has been named HEL1 to indicate the DNA helicase activity of the gene product. HEL1 was identified by screening a lambda gt11 yeast protein expression library with antiserum to purified scHelI. Several independent immunopositive clones were isolated and shown to contain portions of HEL1 either by sequencing or by hybridization to a probe containing HEL1 sequences. The HEL1 open reading frame includes the seven conserved helicase motifs, consistent with the DNA helicase activity of scHelI, and the predicted size of the protein is in agreement with the size of purified scHelI. Partially purified cellular extracts from a hel1 deletion mutant strain did not contain scHelI activity. Homology searches revealed protein sequence homology between HEL1 and two previously identified and biochemically characterized yeast helicases, encoded by the DNA2 and UPF1 genes. Haploid hel1 deletion strains were constructed and shown to be viable with growth rates equivalent to those of parental strains. These strains did not differ from the parental strains in ultraviolet lig...Continue Reading

References

Jun 1, 1992·Chromosoma·P Thömmes, U Hübscher
May 1, 1992·Molecular and Cellular Biology·P LeedsM R Culbertson
Feb 8, 1991·Cell·P S FreemontJ Trowsdale
Jan 1, 1991·Methods in Enzymology·F Sherman, J Hicks
Jan 1, 1991·Methods in Enzymology·F Sherman
Jan 1, 1990·Annual Review of Biochemistry·S W Matson, K A Kaiser-Rogers
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P SungS Prakash
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·M E Budd, J L Campbell
Jan 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S W MatsonJ W George

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