Identification of the gene lon (capR) product as a 94-kilodalton polypeptide by cloning and deletion analysis.

Journal of Bacteriology
J M Schoemaker, A Markovitz

Abstract

A mutation in the lon (capR) gene of Escherichia coli K-12 effects several phenotypic alterations in the mutant cell, such as overproduction of capsular polysaccharide and sensitivity to ultraviolet or ionizing radiation. A previously cloned 9.2-megadalton (Md) EcoRI fragment contained the capR+ gene and specified two polypeptides, 94 kilodaltons (K) and 67K. To provide evidence that the 94K polypeptide is the capR+ gene product, we constructed a capR+ plasmid pJMC40, having a 2.0-Md EcoRI-PstI fragment which codes only for the 94K polypeptide. Plasmids pJMC22 and pJMC30, having deletions of 0.7 and 0.8 Md, respectively, from one end of the 2.0-Md fragment, were also constructed. Each codes for a shortened stable polypeptide (from the 94K). Neither plasmid can confer the capR+ phenotype to capR mutants, confirming that the unaltered 94K polypeptide is the capR+ gene product. Plasmids pJMC51 and pJMC52 each have a deletion of 0.7 Md from the other end of the 2.0-Md fragment, differing only in the orientation of the remaining 1.3-Md fragment with respect to the cloning vehicle. They are nonfunctional with respect to capR+ and do not code for a common polypeptide from the 1.3-Md fragment. These data indicate that the fragments in ...Continue Reading

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Citations

Mar 12, 2010·Protein Science : a Publication of the Protein Society·Javier García-NafríaKeith S Wilson
Apr 1, 1984·Journal of Bacteriology·M F CharetteA Markovitz
Dec 1, 1985·Journal of Bacteriology·M R MauriziS Gottesman
Aug 1, 1983·Journal of Bacteriology·K R Rupprecht, A Markovitz
Jul 1, 1984·Journal of Bacteriology·T A PhillipsF C Neidhardt
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Dec 5, 1982·Journal of Molecular Biology·M F CharetteA Markovitz

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