Identification of the GlialCAM interactome: the G protein-coupled receptors GPRC5B and GPR37L1 modulate megalencephalic leukoencephalopathy proteins.

Human Molecular Genetics
Marta Alonso-GardónRaúl Estévez

Abstract

Megalencephalic Leukoencephalopathy with subcortical Cysts (MLC) is a type of vacuolating leukodystrophy, which is mainly caused by mutations in MLC1 or GLIALCAM. The two MLC-causing genes encode for membrane proteins of yet unknown function that have been linked to the regulation of different chloride channels such as the ClC-2 and VRAC. To gain insight into the role of MLC proteins, we have determined the brain GlialCAM interacting proteome. The proteome includes different transporters and ion channels known to be involved in the regulation of brain homeostasis, proteins related to adhesion or signaling as several G protein-coupled receptors (GPCRs), including the orphan GPRC5B and the proposed prosaposin receptor GPR37L1. Focusing on these two GPCRs, we could validate that they interact directly with MLC proteins. The inactivation of Gpr37l1 in mice upregulated MLC proteins without altering their localization. Conversely, a reduction of GPRC5B levels in primary astrocytes downregulated MLC proteins, leading to an impaired activation of ClC-2 and VRAC. The interaction between the GPCRs and MLC1 was dynamically regulated upon changes in the osmolarity or potassium concentration. We propose that GlialCAM and MLC1 associate with...Continue Reading

References

Apr 17, 1997·Biochemical and Biophysical Research Communications·Z ZengY Li
May 18, 2004·Neurochemistry International·Nathalie RouachChristian Giaume
Nov 25, 2004·Neuroscience·P Kofuji, E A Newman
Nov 30, 2005·Nature Reviews. Molecular Cell Biology·Martin E Hemler
Oct 19, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Biljana DjukicKen D McCarthy
Oct 29, 2008·The Journal of Cell Biology·Olga BarreiroFrancisco Sánchez-Madrid
Mar 29, 2011·Neurobiology of Disease·Anna DuarriRaúl Estévez
May 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ulrike PannaschNathalie Rouach
Jun 1, 2011·Human Molecular Genetics·Tania López-HernándezRaúl Estévez
Aug 16, 2011·Biochemical and Biophysical Research Communications·Takamitsu SanoYoshio Hirabayashi
Oct 19, 2011·Brain : a Journal of Neurology·Margreet C RidderMarjo S van der Knaap
Nov 10, 2011·Molecular & Cellular Proteomics : MCP·Wolfgang BildlUwe Schulte
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 22, 2012·Science Signaling·Yeon-Jeong KimYoshio Hirabayashi
May 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rebecca C MeyerRandy A Hall
Sep 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Daniela MarazzitiGlauco P Tocchini-Valentini
Dec 29, 2013·The Journal of Biological Chemistry·Ebba Gregorsson LundiusPer Svenningsson
Jan 15, 2014·Current Biology : CB·Sang-Ho KwonKeith E Mostov
Aug 19, 2014·Brain Research·Rebecca C MeyerRandy A Hall
Jan 23, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gina La SalaGlauco P Tocchini-Valentini
Nov 18, 2015·Journal of Visualized Experiments : JoVE·Anne-Cécile BoulayMartine Cohen-Salmon
Oct 18, 2016·Glia·Shouta SugioKenji F Tanaka
Mar 11, 2017·Neuroscience and Biobehavioral Reviews·Alba Bellot-SaezYossi Buskila
Mar 17, 2017·Molecular Systems Biology·Kate SokolinaIgor Stagljar
Apr 22, 2017·Frontiers in Cell and Developmental Biology·Christina M Termini, Jennifer M Gillette
Sep 15, 2017·The Journal of Physiology·Héctor Gaitán-PeñasRaúl Estévez
Nov 22, 2017·Neuroscience Research·Yasuyuki ItoMichihiro Igarashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.