Identification of the heptameric Lsm complex that binds U6 snRNA in Trypanosoma brucei

Molecular and Biochemical Parasitology
Itai Dov TkaczShulamit Michaeli

Abstract

Lsm proteins are ubiquitous, multifunctional proteins that are involved in nuclear processing and turnover of many RNAs in eukaryotes. Lsm proteins form two distinct complexes, the Lsm2-8 complex, which binds U6 snRNA, and the Lsm1-7 complex, which governs mRNA degradation. Previously, seven Lsm proteins were identified in Trypanosoma brucei. Two of these proteins were later identified as SSm proteins (specific spliceosomal Sm proteins). In this study, the Lsm proteins (Lsm2 and Lsm5) that bind to U6 snRNA were identified. RNAi silencing and protein purification of TAP-tagged Lsm proteins were used to identify all the components of the trypanosome heptameric Lsm2-8 complex. Localization studies demonstrated that these proteins are found in the nucleus, near the nucleolus. Lsm proteins were not detected in cytoplasmic bodies that were tagged with YFP-Dhh1, which may suggest that in trypanosomes, Lsm-mediated degradation is not confined to such bodies.

References

Aug 2, 1996·The Journal of Biological Chemistry·J D BangsJ L Roggy
Jan 27, 1999·Bioinformatics·S R Eddy
Jun 16, 1999·The EMBO Journal·J Salgado-GarridoB Séraphin
Apr 4, 2000·The EMBO Journal·E BouveretB Séraphin
Jul 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z PalfiA Bindereif
May 10, 2001·Current Opinion in Cell Biology·C L Will, R Lührmann
May 25, 2002·Molecular and Cellular Biology·Nenad Tomasevic, Brenda A Peculis
Nov 20, 2002·The Journal of Biological Chemistry·Joanna KufelDavid Tollervey
Mar 3, 2004·The Journal of Biological Chemistry·Qing LiuShulamit Michaeli
Apr 13, 2004·Molecular Biology of the Cell·Cesar F FernandezSandra L Wolin
Sep 16, 2006·The EMBO Journal·Pingping WangAlbrecht Bindereif
Dec 22, 2006·Nature Reviews. Molecular Cell Biology·Ana EulalioElisa Izaurralde
Mar 10, 2007·EMBO Reports·Yaniv LustigShulamit Michaeli
Mar 14, 2007·Molecular Cell·Roy Parker, Ujwal Sheth
Apr 3, 2007·Biochemical and Biophysical Research Communications·Fabíola Barbieri HoletzSamuel Goldenberg
Jul 20, 2007·Molecular Microbiology·Alejandro CassolaAlberto C Frasch

❮ Previous
Next ❯

Citations

Jul 2, 2010·The Journal of Biological Chemistry·Itai Dov TkaczShulamit Michaeli
Jan 4, 2013·The Journal of Biological Chemistry·Sachin Kumar GuptaShulamit Michaeli
Apr 27, 2013·Nucleic Acids Research·Anna GoliszJoanna Kufel
Jul 17, 2009·Genes & Development·Zsofia PalfiAlbrecht Bindereif
May 12, 2009·Eukaryotic Cell·Daniela Luz AmbrósioArthur Günzl
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Jan-Peter DanielsBill Wickstead
Dec 20, 2014·Molecular Microbiology·Daniela L AmbrósioArthur Günzl
Jul 21, 2017·PloS One·Carina GoosSusanne Kramer
Mar 17, 2016·World Journal of Biological Chemistry·María Albertina RomaniukAlejandro Cassola
Feb 2, 2011·RNA Biology·Nicolas JaéAlbrecht Bindereif
Jun 25, 2019·Wiley Interdisciplinary Reviews. RNA·Caroline VindryNancy Standart
Oct 23, 2018·Wiley Interdisciplinary Reviews. RNA·Susanne Kramer, Alexander G McLennan

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.