Abstract
Lsm proteins are ubiquitous, multifunctional proteins that are involved in nuclear processing and turnover of many RNAs in eukaryotes. Lsm proteins form two distinct complexes, the Lsm2-8 complex, which binds U6 snRNA, and the Lsm1-7 complex, which governs mRNA degradation. Previously, seven Lsm proteins were identified in Trypanosoma brucei. Two of these proteins were later identified as SSm proteins (specific spliceosomal Sm proteins). In this study, the Lsm proteins (Lsm2 and Lsm5) that bind to U6 snRNA were identified. RNAi silencing and protein purification of TAP-tagged Lsm proteins were used to identify all the components of the trypanosome heptameric Lsm2-8 complex. Localization studies demonstrated that these proteins are found in the nucleus, near the nucleolus. Lsm proteins were not detected in cytoplasmic bodies that were tagged with YFP-Dhh1, which may suggest that in trypanosomes, Lsm-mediated degradation is not confined to such bodies.
References
Jul 1, 1992·Molecular and Cellular Biology·M P TernsJ E Dahlberg
Mar 1, 1989·Molecular and Cellular Biology·J MottramR G Nelson
Aug 2, 1996·The Journal of Biological Chemistry·J D BangsJ L Roggy
Jan 27, 1999·Bioinformatics·S R Eddy
Jun 16, 1999·The EMBO Journal·J Salgado-GarridoB Séraphin
Aug 3, 1999·The EMBO Journal·A E MayesJ D Beggs
Oct 16, 1999·The EMBO Journal·T AchselR Lührmann
Apr 4, 2000·The EMBO Journal·E BouveretB Séraphin
Jul 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z PalfiA Bindereif
Oct 3, 2000·The Journal of Biological Chemistry·Z WangP T Englund
May 10, 2001·Current Opinion in Cell Biology·C L Will, R Lührmann
May 25, 2002·Molecular and Cellular Biology·Nenad Tomasevic, Brenda A Peculis
Jun 22, 2002·Molecular and Cellular Biology·Joanna KufelDavid Tollervey
Nov 20, 2002·The Journal of Biological Chemistry·Joanna KufelDavid Tollervey
May 6, 2003·Science·Ujwal Sheth, Roy Parker
Sep 17, 2003·Genes & Development·Ramesh S PillaiDaniel Schümperli
Oct 9, 2003·The Journal of Biological Chemistry·Michal MandelboimShulamit Michaeli
Mar 3, 2004·The Journal of Biological Chemistry·Qing LiuShulamit Michaeli
Mar 12, 2004·International Journal for Parasitology·Shai UlielShulamit Michaeli
Apr 13, 2004·Molecular Biology of the Cell·Cesar F FernandezSandra L Wolin
Apr 21, 2005·RNA·Xue-Hai LiangShulamit Michaeli
Sep 24, 2005·Journal of Cell Science·Yaniv LustigShulamit Michaeli
Sep 16, 2006·The EMBO Journal·Pingping WangAlbrecht Bindereif
Dec 22, 2006·Nature Reviews. Molecular Cell Biology·Ana EulalioElisa Izaurralde
Mar 10, 2007·EMBO Reports·Yaniv LustigShulamit Michaeli
Mar 14, 2007·Molecular Cell·Roy Parker, Ujwal Sheth
Apr 3, 2007·Biochemical and Biophysical Research Communications·Fabíola Barbieri HoletzSamuel Goldenberg
May 22, 2007·Molecular and Biochemical Parasitology·Steven KellyMark Carrington
Jul 20, 2007·Molecular Microbiology·Alejandro CassolaAlberto C Frasch
Citations
Jul 2, 2010·The Journal of Biological Chemistry·Itai Dov TkaczShulamit Michaeli
Jan 4, 2013·The Journal of Biological Chemistry·Sachin Kumar GuptaShulamit Michaeli
Feb 18, 2010·Nucleic Acids Research·Hadassa ShakedShulamit Michaeli
Apr 27, 2013·Nucleic Acids Research·Anna GoliszJoanna Kufel
May 15, 2013·Nucleic Acids Research·Sachin Kumar GuptaShulamit Michaeli
Jul 17, 2009·Genes & Development·Zsofia PalfiAlbrecht Bindereif
May 12, 2009·Eukaryotic Cell·Daniela Luz AmbrósioArthur Günzl
Jun 29, 2010·Eukaryotic Cell·Arthur Günzl
Nov 26, 2008·Eukaryotic Cell·Avraham HuryShulamit Michaeli
Dec 24, 2008·Molecular and Cellular Biology·Jesse R ZamudioDavid A Campbell
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Jan-Peter DanielsBill Wickstead
Feb 4, 2010·Journal of Cell Science·Susanne KramerMark Carrington
May 5, 2012·Genetics·Guy M L PerrySteven J Scheinman
Apr 30, 2011·Future Microbiology·Shulamit Michaeli
Mar 26, 2013·FEBS Letters·Sachin Kumar GuptaShulamit Michaeli
Dec 20, 2014·Molecular Microbiology·Daniela L AmbrósioArthur Günzl
Jun 13, 2014·RNA Biology·Sachin Kumar GuptaShulamit Michaeli
Mar 23, 2017·RNA·Kirsten A ReimerStephen D Rader
Jun 20, 2017·PLoS Pathogens·Susanne Kramer
Jul 21, 2017·PloS One·Carina GoosSusanne Kramer
Mar 17, 2016·World Journal of Biological Chemistry·María Albertina RomaniukAlejandro Cassola
Feb 2, 2011·RNA Biology·Nicolas JaéAlbrecht Bindereif
Jun 25, 2019·Wiley Interdisciplinary Reviews. RNA·Caroline VindryNancy Standart
Oct 23, 2018·Wiley Interdisciplinary Reviews. RNA·Susanne Kramer, Alexander G McLennan