PMID: 8590483Sep 1, 1995Paper

Identification of the heterothallic mutation in HO-endonuclease of S. cerevisiae using HO/ho chimeric genes

Current Genetics
H MeironDina Raveh

Abstract

HO-endonuclease initiates a mating-type switch in the yeast S. cerevisiae by making a double-strand cleavage in the DNA of the mating-type gene, MAT. Heterothallic strains of yeast have a stable mating type and contain a recessive ho allele. Here we report the sequence of the ho allele; ho has four point mutations all of which encode for substitute amino acids. The fourth mutation is a leucine to histidine substitution within a presumptive zinc finger. Chimeric HO/ho genes were constructed in vivo by converting different parts of the sequence of the genomic ho allele to the HO sequence by gene conversion. HO activity was assessed by three bioassays: a mating-type switch, extinction of expression of an a-specific reporter gene, and the appearance of Canr Ade- papillae resulting from excision of an engineered Ty element containing the HO-endonuclease target site and a SUP4 degrees gene. We found that the replacement of the fourth point mutation in ho to the HO sequence restored HO activity to the chimeric endonuclease.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M H LittleN D Hastie
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M F HoekstraF Heffron
Jan 1, 1990·Biotherapy·W E Fibbe, J H Falkenburg
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J A NickoloffF Heffron
Dec 1, 1986·Molecular and Cellular Biology·D W RussellI Herskowitz
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·C T Caskey
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M Berg
Jun 17, 1988·Science·J S Richardson, D C Richardson
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K L Wilson, I Herskowitz
Jan 1, 1986·Human Genetics·G F De Stefano, L Ferrucci
Jan 1, 1984·Cold Spring Harbor Symposia on Quantitative Biology·R Kostriken, F Heffron
Jan 1, 1983·Methods in Enzymology·T L Orr-WeaverR J Rothstein
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R JensenI Herskowitz
May 17, 1994·Biochemistry·A C JamiesonJ A Wells

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Citations

Sep 9, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takeshi AkaoHitoshi Shimoi
Oct 9, 2009·Genome Research·Juan Lucas ArguesoGonçalo A G Pereira
Aug 11, 2007·Bioscience, Biotechnology, and Biochemistry·Yukio TamaiYoshinobu Kaneko
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Patrick A GibneyDavid Botstein
Jul 1, 2017·PLoS Computational Biology·Shaohuan WuWenfeng Qian
Oct 25, 2017·Molecular and Cellular Biology·Lina HeistingerDiethard Mattanovich
Aug 25, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Michael Knop
Jun 19, 2014·G3 : Genes - Genomes - Genetics·Lisa SolieriStefano Cassanelli
Mar 14, 1997·The Journal of Biological Chemistry·Y JinD Norris
Mar 25, 2017·Scientific Reports·Indu KhatriSrikrishna Subramanian
Mar 12, 2018·Current Genetics·Yaniv HarariMartin Kupiec
Oct 9, 1999·Journal of Bacteriology·G MarinoniJ Piskur
Nov 17, 2020·Yeast·Gilles FischerBertrand Llorente
Apr 9, 2021·Current Genetics·Laetitia Maroc, Cécile Fairhead
Mar 17, 1999·Methods : a Companion to Methods in Enzymology·C P Liang, W T Garrard
Sep 17, 2003·Genes & Development·Warren P Voth, David J Stillman
Nov 1, 2021·FEMS Yeast Research·Julia L Crunden, Stephanie Diezmann

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