Identification of the initial molecular changes in response to circulating angiogenic cells-mediated therapy in critical limb ischemia.

Stem Cell Research & Therapy
Lucia Beltran-CamachoMª Carmen Durán-Ruiz

Abstract

Critical limb ischemia (CLI) constitutes the most aggressive form of peripheral arterial occlusive disease, characterized by the blockade of arteries supplying blood to the lower extremities, significantly diminishing oxygen and nutrient supply. CLI patients usually undergo amputation of fingers, feet, or extremities, with a high risk of mortality due to associated comorbidities. Circulating angiogenic cells (CACs), also known as early endothelial progenitor cells, constitute promising candidates for cell therapy in CLI due to their assigned vascular regenerative properties. Preclinical and clinical assays with CACs have shown promising results. A better understanding of how these cells participate in vascular regeneration would significantly help to potentiate their role in revascularization. Herein, we analyzed the initial molecular mechanisms triggered by human CACs after being administered to a murine model of CLI, in order to understand how these cells promote angiogenesis within the ischemic tissues. Balb-c nude mice (n:24) were distributed in four different groups: healthy controls (C, n:4), shams (SH, n:4), and ischemic mice (after femoral ligation) that received either 50 μl physiological serum (SC, n:8) or 5 × 105 hum...Continue Reading

References

Dec 1, 1994·Journal of Leukocyte Biology·J A Smith
Jun 18, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Emmanouil Chavakis, Stefanie Dimmeler
Jun 11, 2004·Journal of Cell Science·Xuan ZhouG Ian Gallicano
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adil I KhanPaul Kubes
Jan 8, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·James G Tidball
Oct 29, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Anne-Louise HemdahlGöran K Hansson
Jul 4, 2006·The American Journal of Pathology·Gaoyuan CaoHorace M Delisser
Jul 19, 2006·Canadian Journal of Physiology and Pharmacology·Maziar RahmaniBruce M McManus
Jan 16, 2007·Journal of Vascular Surgery·L NorgrenUNKNOWN TASC II Working Group
Mar 30, 2007·Leukemia·D N PraterM C Yoder
Jun 21, 2007·The Journal of Biological Chemistry·Ulrich J H SachsSentot Santoso
Dec 15, 2007·The American Journal of Pathology·Sarah NolanVictoria Ridger
Mar 20, 2008·Cardiovascular Research·Anna ZampetakiQingbo Xu
Apr 1, 2008·Annals of Surgery·Ralf W SprengersMarianne C Verhaar
Sep 27, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Surovi HazarikaBrian H Annex
Feb 21, 2009·Journal of Visualized Experiments : JoVE·Hiroshi NiiyamaJohn P Cooke
Apr 22, 2009·Journal of Cellular and Molecular Medicine·F SabatierF Dignat-George
Jun 2, 2012·Frontiers in Bioscience (Landmark Edition)·Fuyong DuXiao-Feng Yang
Sep 12, 2012·European Journal of Immunology·Andrea SchrollGuenter Weiss
Oct 26, 2012·The FEBS Journal·Leyuan BaoSreenivasan Ponnambalam
Nov 7, 2012·Angiology·Geoffrey O OumaMoshe Y Flugelman
Jul 23, 2013·Nature·Xiaomeng WangJohn Greenwood
Nov 2, 2014·Protein & Cell·Qing XieWeizhou Zhang
Mar 31, 2015·Seminars in Vascular Surgery·Sae Hee Ko, Dennis F Bandyk
Apr 25, 2015·Circulation Research·John P Cooke, Douglas W Losordo
May 21, 2015·International Journal of Molecular Sciences·Smriti Murali KrishnaJonathan Golledge

❮ Previous
Next ❯

Citations

Jul 30, 2020·International Journal of Molecular Sciences·Sara Eslava-AlconMª Carmen Durán-Ruiz
Dec 29, 2020·Frontiers in Cell and Developmental Biology·Marta Rojas-TorresMª Carmen Durán-Ruiz
Mar 4, 2021·International Journal of Molecular Sciences·Lucía Beltrán-CamachoMᵃ Carmen Durán-Ruiz

❮ Previous
Next ❯

Methods Mentioned

BETA
amputation
flow cytometry
PCA
environmental stresses

Software Mentioned

Venny
IPA
Mascot
GraphPad Prism
Proteome Discoverer
ProteomeDiscoverer
Perseus
Rad CFX manager
Bio
Zen

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.