PMID: 9435243Jan 22, 1998Paper

Identification of the lactococcal exonuclease/recombinase and its modulation by the putative Chi sequence

Proceedings of the National Academy of Sciences of the United States of America
Meriem El KarouiAlexandra Gruss

Abstract

Studies of RecBCD-Chi interactions in Escherichia coli have served as a model to understand recombination events in bacteria. However, the existence of similar interactions has not been demonstrated in bacteria unrelated to E. coli. We developed an in vivo model to examine components of dsDNA break repair in various microorganisms. Here, we identify the major exonuclease in Lactococcus lactis, a Gram-positive organism evolutionarily distant from E. coli, and provide evidence for exonuclease-Chi interactions. Insertional mutants of L. lactis, screened as exonuclease-deficient, affected a single locus and resulted in UV sensitivity and recombination deficiency. The cloned lactococcal genes (called rexAB) restored UV resistance, recombination proficiency, and the capacity to degrade linear DNA, to an E. coli recBCD mutant. In this context, DNA degradation is specifically blocked by the putative lactococcal Chi site (5'-GCGCGTG-3'), but not by the E. coli Chi (5'-GCTGGTGG-3') site. RexAB-mediated recombination was shown to be stimulated approximately 27-fold by lactococcal Chi. Our results reveal that RexAB fulfills the biological roles of RecBCD and indicate that its activity is modulated by a short DNA sequence. We speculate that...Continue Reading

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Citations

Dec 22, 1999·Mutation Research·J A Eisen, P C Hanawalt
Feb 15, 2000·Research in Microbiology·M El KarouiA Gruss
Dec 23, 1999·Current Opinion in Genetics & Development·I KobayashiI Uchiyama
Apr 8, 2000·Trends in Biochemical Sciences·S C Kowalczykowski
Dec 18, 2010·Nature Reviews. Microbiology·Fabrice TouzainMeriem El Karoui
Jul 25, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M El KarouiA Gruss
May 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Naofumi HandaStephen C Kowalczykowski
Jul 3, 2009·Genes & Development·Hengyao NiuPatrick Sung
Dec 13, 2006·Journal of Bacteriology·Zhi LiuPatrik M Bavoil
Mar 29, 2000·Journal of Bacteriology·N HandaI Kobayashi
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Mark S Dillingham, Stephen C Kowalczykowski
Sep 1, 2005·PLoS Genetics·Eduardo P C RochaBénédicte Michel
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Marc PrudhommeJean-Pierre Claverys
May 22, 2007·Molecular Microbiology·Sarah BigotFrançois Cornet
Jan 30, 2013·Biochemical Society Transactions·John K BlackwoodNicholas P Robinson
Jun 23, 2016·Nucleic Acids Research·Andrew F TaylorGerald R Smith
Apr 25, 2006·The Journal of Biological Chemistry·Frédéric ChédinStephen C Kowalczykowski
Jul 1, 2009·Critical Reviews in Microbiology·Agnieszka K Szczepańska
Jul 17, 2004·Microbiology·David HalpernMeriem El-Karoui
Apr 11, 2000·Journal of Molecular Biology·F ChédinS C Kowalczykowski

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