PMID: 9420301Jan 7, 1998Paper

Identification of the leader-body junctions for the viral subgenomic mRNAs and organization of the simian hemorrhagic fever virus genome: evidence for gene duplication during arterivirus evolution

Journal of Virology
E K GodenyR J de Groot

Abstract

Simian hemorrhagic fever virus (SHFV) was recently reclassified and assigned to the new virus family Arteriviridae. During replication, arteriviruses produce a 3' coterminal, nested set of subgenomic mRNAs (sgRNAs). These sgRNAs arise by discontinuous transcription, and each contains a 5' leader sequence which is joined to the body of the mRNA through a conserved junction sequence. Only the 5'-most open reading frame (ORF) is believed to be transcribed from each sgRNA. The SHFV genome encodes nine ORFs that are presumed to be expressed from sgRNAs. However, reverse transcription-PCR analysis with leader- and ORF-specific primers identified only eight sgRNA species. The consensus sequence 5'-UCNUUAACC-3' was identified as the junction motif. Our data suggest that sgRNA 2 may be bicistronic, expressing both ORF 2a and ORF 2b. SHFV encodes three more ORFs on its genome than the other arteriviruses. Comparative sequence analysis suggested that SHFV ORFs 2a, 2b, and 3 are related to ORFs 2 through 4 of the other arteriviruses. Evidence which suggests that SHFV ORFs 4 through 6 are related to ORFs 2a through 3 and may have resulted from a recombination event during virus evolution is presented.

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Citations

Aug 14, 2013·The Journal of General Virology·Eric J SnijderYing Fang
Nov 27, 2015·Archives of Virology·Jens H KuhnTony L Goldberg
Feb 11, 2011·The Journal of General Virology·Andrew E FirthClara C Posthuma
Oct 19, 2012·Journal of Virology·Michael LauckTony L Goldberg
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Han DiMargo A Brinton
Jul 10, 1999·Journal of Virology·E J SnijderA A de Vries
Jan 9, 2021·Biochemical and Biophysical Research Communications·Anastasia A Gulyaeva, Alexander E Gorbalenya
Jun 1, 2001·Virology·A A de VriesP J Rottier

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