Identification of the major phosphorylation sites for protein kinase C in kit/stem cell factor receptor in vitro and in intact cells.

The Journal of Biological Chemistry
P Blume-JensenL Rönnstrand

Abstract

The c-kit-encoded tyrosine kinase receptor for stem cell factor (Kit/SCFR) is crucial for the development of hematopoietic cells, melanoblasts, and germ cells. Ligand stimulation of Kit/SCFR leads to receptor dimerization and autophosphorylation on tyrosine residues. We recently showed, that protein kinase C (PKC) acts in an SCF-stimulated negative feedback loop, which controls Kit/SCFR tyrosine kinase activity and modulates the cellular responses to SCF (Blume-Jensen, P., Siegbahn, A., Stabel, S., Heldin, C.-H., and Rönnstrand, L. (1993) EMBO J. 12, 4199-4209). We present here the identification of the major phosphorylation sites for PKC in Kit/SCFR. Two serine residues in the kinase insert, Ser-741 and Ser-746, are PKC-dependent phosphorylation sites in vivo and account for all phosphorylation by PKC in vitro. Together they comprise more than 60% of the total SCF-stimulated receptor phosphorylation in living cells and 85-90% of its phosphorylation in resting cells. Two additional serine residues, Ser-821 close to the major tyrosine autophosphorylation site in the kinase domain and Ser-959 in the carboxyl terminus are SCF-stimulated PKC-dependent phosphorylation sites. However, they are not phosphorylated directly by PKC-alpha...Continue Reading

References

Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S LevY Yarden
Mar 1, 1992·Cellular Signalling·R V FareseD R Cooper
Mar 15, 1989·Biochemical and Biophysical Research Communications·E KobayashiT Tamaoki
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R H AebersoldS B Kent
Jan 16, 1985·Biochemical and Biophysical Research Communications·K MiyazonoF Takaku
Feb 1, 1994·Trends in Biochemical Sciences·L V Dekker, P J Parker

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Citations

Mar 10, 1998·Molecular Reproduction and Development·H FengA Sandra
Mar 24, 2004·Leukemia Research·Martin Sattler, Ravi Salgia
Dec 3, 1999·The International Journal of Biochemistry & Cell Biology·D Linnekin
Feb 8, 2003·Biochimica Et Biophysica Acta·Beston F NoreC I Edvard Smith
Feb 19, 2004·The Journal of Experimental Medicine·Maria Alvarado-KristenssonTommy Andersson
Sep 6, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Amanda J Redig, Leonidas C Platanias
Nov 20, 2002·Journal of Virology·Pietri PuustinenKristiina Mäkinen
Dec 31, 2004·Stem Cells·Johan LennartssonR Shivakrupa
Feb 21, 2014·Journal of Molecular Histology·Xuejiu WangZhipeng Fan
May 27, 2004·Expert Opinion on Therapeutic Targets·Lolita Banerji, Martin Sattler
Jan 15, 2013·Trends in Biochemical Sciences·Lauren E Locascio, Daniel J Donoghue
Jun 7, 2011·Leukemia Research·Celalettin UstunCem Akin
Aug 12, 2008·Seminars in Oncology·Claudia SchollStefan Fröhling
Apr 28, 2006·The FEBS Journal·Heike RebholzIvan T Gout
Feb 18, 2006·European Journal of Pharmacology·Laurent ReberNelly Frossard
Jul 26, 2000·Hematology/oncology Clinics of North America·M L Taylor, D D Metcalfe
Jul 31, 2012·Biotechnology Advances·Jeong-Hun KangYoshiki Katayama
Mar 19, 1999·Methods : a Companion to Methods in Enzymology·H Avraham, D J Price
Jun 1, 1999·FEBS Letters·S ValgeirsdóttirC H Heldin
Jun 27, 2008·Neuromuscular Disorders : NMD·Jean-Marc BurgunderErwin Sigel
Aug 15, 2000·The Journal of Biological Chemistry·M von LindernB Löwenberg

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