Identification of the metalloproteinase stromelysin in the physis

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Ann L ArmstrongM G Ehrlich

Abstract

For long bone growth to occur, calcification of the matrix must commence in the lower hypertrophic zone of the growth plate. It is generally accepted that physeal proteoglycans help regulate mineralization, and that at least in vitro, intact proteoglycans can inhibit mineralization. Thus degradation of proteoglycan may be a necessary step prior to calcification. Previous work in our laboratory has demonstrated the presence of neutral metallo-proteases in the growth plate with highest levels in the hypertrophic zone, where calcification occurs. Stromelysin (MMP-3) is a connective tissue matrix-degrading enzyme. It was formerly known as proteoglycanase and is generally considered to be one of the major proteoglycan degrading enzymes in cartilage. Stromelysin is implicated in cartilage destruction in osteoarthritis and may also be involved in tissue remodeling in the physis. Our goal was to determine if the neutral protease previously reported by the authors in the physis was stromelysin. In this study we used Western blots and antibodies to stromelysin and to the stromelysin cleavage site in aggrecan, the most common form of proteoglycan, to demonstrate the presence of stromelysin in the bovine physis. When an antibody raised aga...Continue Reading

References

Jan 1, 1975·Connective Tissue Research·J Dunstone, S Cleland
May 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A L BoskeyM G Ehrlich
Jan 1, 1985·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M G EhrlichH J Mankin
Jun 1, 1989·The Anatomical Record·A R PooleE R Lee
Aug 1, 1985·The Journal of Clinical Investigation·D D DeanD S Howell
Oct 1, 1987·Calcified Tissue International·J A BuckwalterR Ungar
Jan 1, 1987·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J A BuckwalterH J Mankin
Jan 1, 1984·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M G EhrlichH J Mankin
Jan 1, 1984·Metabolic Bone Disease & Related Research·J P Scherft, S Moskalewski
Oct 6, 1997·Archives of Biochemistry and Biophysics·T M HeringT D Huynh
Jan 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J AlvarezJ M López
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z ZhouK Tryggvason
Apr 20, 2000·Journal of Cellular Biochemistry·M D'AngeloP S Leboy

❮ Previous
Next ❯

Citations

Apr 2, 2004·European Journal of Oral Sciences·Stephanie FanchonMichel Goldberg
Nov 6, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Marco A da SilvaHelmtrud I Roach
Oct 24, 2009·Journal of Applied Physiology·Adi ReichEfrat Monsonego Ornan
Jan 4, 2007·The Journal of Bone and Joint Surgery. American Volume·Mark C LeeQian Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.