Identification of the minimal replicon of plasmid pMEA300 of the methylotrophic actinomycete Amycolatopsis methanolica

Molecular Microbiology
J W VrijbloedLubbert Dijkhuizen

Abstract

The actinomycete Amycolatopsis methanolica contains a 13.3 kb plasmid (pMEA300), capable of enhancing the spontaneous mutation frequency of its host. Depending on the growth medium pMEA300 is not only maintained as an integrated element but can additionally be present as a multicopy, autonomously replicating plasmid. The minimal replicon of pMEA300 was identified. Two unlinked DNA fragments of 2.6 kb and 0.8 kb were required for pMEA300 maintenance. Sequence analysis of the 2.6 kb fragment revealed at least two open reading frames, orfA and orfB, encoding putative proteins of 170 amino acids (18,373 Da) and 416 amino acids (45,260 Da), respectively. No clear similarities were found between the deduced amino acid sequences of the putative orfA and orfB products of pMEA300 and replication proteins identified for various Streptomyces plasmids. The pMEA300 proteins of A. methanolica thus may represent unfamiliar types. The 0.8 kb fragment contained a single complete open reading frame (korA), encoding a protein of 118 amino acids (12,917 Da). The putative KorA protein of pMEA300 shows sequence similarity with various other Streptomyces plasmid-encoded Kor proteins which may belong to the GntR family of transcriptional repressor pro...Continue Reading

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Citations

Mar 1, 2007·Indian Journal of Microbiology·S Malhotra, R Lal
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Elisabeth GrohmannManuel Espinosa
Jun 5, 2008·Antonie van Leeuwenhoek·Evelien M te PoeleLubbert Dijkhuizen
Apr 11, 2003·Journal of Industrial Microbiology & Biotechnology·Gauri DhingraRup Lal
Jun 17, 1998·FEMS Microbiology Letters·N Vukov, B Vasiljevic
Apr 1, 1997·Applied and Environmental Microbiology·D CueM C Flickinger

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