Identification of the minimal repression domain of SUPERMAN shows that the DLELRL hexapeptide is both necessary and sufficient for repression of transcription in Arabidopsis

Biochemical and Biophysical Research Communications
Keiichiro HiratsuM Ohme-Takagi

Abstract

We reported previously that the carboxy-terminal 30 amino acids of SUPERMAN (SUPRD) function as a repression domain in Arabidopsis. In this study, we identified the peptide sequences in SUPRD that is both necessary and sufficient for repression of transcription. To our surprise, the hexapeptide DLELRL was sufficient, by itself, to confer the ability to repress transcription on a DNA-binding domain. A database search revealed that there are 32 TFIIIA-type zinc finger proteins in the Arabidopsis genome that contain a hexapeptide sequence similar or identical to that of DLELRL. These peptides acted as repression domains, suggesting that these zinc finger proteins might function as active repressors. Further mutational analysis within DLELRL revealed that an amphiphilic motif composed of six amino acids (XLxLXL) with preferences at the first and fifth positions is necessary and sufficient for strong repression. An assay of positional effects suggested that GAL4DB-DLELRL might function as a short-range repressor. A possible mechanism of the DLELRL-mediated repression is discussed.

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Citations

Nov 22, 2007·Molecular Genetics and Genomics : MGG·Chang-Jie JiangHiroshi Takatsuji
Mar 28, 2009·Plant & Cell Physiology·Miho Ikeda, Masaru Ohme-Takagi
May 30, 2009·Plant & Cell Physiology·Nobutaka Mitsuda, Masaru Ohme-Takagi
Jan 13, 2006·Plant Physiology·Toshitsugu NakanoHideaki Shinshi
Nov 9, 2011·Plant Physiology·Barry CausierBrendan Davies
Oct 28, 2008·BMC Evolutionary Biology·Yves DeveauxAlain Lecharny
Aug 12, 2010·Development·Andrea GallavottiRobert J Schmidt
Jan 8, 2008·Bioscience, Biotechnology, and Biochemistry·Tetsuya ChujoHisakazu Yamane
May 8, 2014·Journal of Experimental Botany·Gang LiangDiqiu Yu
Feb 14, 2012·Plant Science : an International Journal of Experimental Plant Biology·Agnieszka Kiełbowicz-Matuk
Jun 21, 2011·Plant Science : an International Journal of Experimental Plant Biology·Yukihiro ItoNori Kurata
Jul 8, 2008·Trends in Plant Science·Vivian F Irish

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