PMID: 6170943May 11, 1981Paper

Identification of the modified nucleotides produced by covalent photoaddition of hydroxymethyltrimethylpsoralen to RNA

Nucleic Acids Research
J P BachellerieJ E Hearst

Abstract

The reaction between RNA and 4'hydroxymethyl-4,5',8-trimethylpsoralen has been studied. Both natural RNA and synthetic RNAs were used. The base specificity of the reaction was found to be the same in natural RNA, homopolymers, and mononucleotides. Uridine was found to be the most reactive base in all cases. The kinetics of formation and reversal of monoadducts and crosslinks has been examined. Paper electrophoretic conditions are described which provide a separation of the monoaddition and crosslinked photoproducts. The relative and absolute amounts of monoadducts and crosslinks can be determined very accurately with this system. Paper electrophoresis provides good separations of the different photoproducts. The mobilities of the products are a simple function of their molecular weights and charges.

References

Nov 25, 1979·Journal of Molecular Biology·P WollenzienC R Cantor
Oct 10, 1979·Nucleic Acids Research·D Rabin, D M Crothers
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P L WollenzienJ E Hearst
Jul 2, 1976·Science·C V HansonJ E Hearst
Sep 1, 1965·Journal of Molecular Biology·F SangerB G Barrell
Nov 19, 1969·Biochimica Et Biophysica Acta·M A Pathak, D M Krämer

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Citations

Apr 1, 1983·Cell·J F Thompson, J E Hearst
May 11, 1982·Nucleic Acids Research·S TurnerH F Noller
Nov 4, 1985·European Journal of Biochemistry·P E Nielsen, V Leick
Mar 1, 1987·Photochemistry and Photobiology·N M Calvin, P C Hanawalt
Jan 1, 1982·Analytical Biochemistry·D C Youvan, J E Hearst

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