PMID: 11606220Oct 19, 2001Paper

Identification of the molecular interaction site of amyloid beta peptide by using a fluorescence assay

The Journal of Peptide Research : Official Journal of the American Peptide Society
K WatanabeH Okuno

Abstract

Beta-amyloid peptides (Abeta) are the main protein components of neuritic plaques and are important in the pathogenesis of Alzheimer's disease. It is reported that Abeta itself is not toxic; however, it becomes toxic to neuronal cells once it has aggregated into amyloid fibrils by peptide-peptide interactions. In this study, to specify the molecular mechanism of aggregation, a novel fluorescence assay was designed. For this purpose, possible partial peptides (38 types of 5-mer) were synthesized on solid-phase. The molecular interactions were examined by a fluorescence probe possessing Lys-Leu-Val-Phe-Phe (KLVFF) as a molecular recognition site. KLVFF is known to be a minimum sequence for formation of the Abeta aggregate. A specific interaction was observed between labeled and immobilized KLVFF. It suggests that the aggregation of Abeta was controlled by the recognition of KLVFF itself by hydrophobic and electrostatic interactions.

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Citations

Dec 28, 2011·Analytical and Bioanalytical Chemistry·Ken-ichi WatanabeMasashi Yabuki
May 27, 2014·Molecular and Cellular Neurosciences·Ryan T CameronGeorge S Baillie
Apr 5, 2011·Chemical Biology & Drug Design·Ashley A Reinke, Jason E Gestwicki
May 29, 2018·Chembiochem : a European Journal of Chemical Biology·Purnandhu BoseKentaro Tashiro
Nov 4, 2009·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Yong Hoon ParkHyun Duk Yang
Nov 7, 2019·Pharmaceutics·Francesca PederzoliAndreas M Grabrucker
Dec 11, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·S AkikusaT Konakahara
Mar 20, 2016·The Biochemical Journal·Marten VillmowCordelia Schiene-Fischer
Sep 15, 2012·Chemical & Pharmaceutical Bulletin·Hideharu SuzukiMasamichi Nakakoshi
May 3, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Ming-Shen LinWen-Yih Chen
Jan 16, 2003·Bioconjugate Chemistry·Guobao ZhangStanley Stein

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