Identification of the N-linked oligosaccharide sites in chick corneal lumican and keratocan that receive keratan sulfate.

The Journal of Biological Chemistry
J R DunlevyJ R Hassell

Abstract

Corneal proteoglycans have chondroitin/dermatan and keratan sulfate (KS) chains and belong to the leucine-rich proteoglycan gene family. Corneal KS is N-linked to Asn of an NX(S/T) site through a complex oligosaccharide linkage region. Only some sites receive KS, whereas others remain in a high mannose form. To determine whether the attachment of KS was biased toward specific sites, we isolated trypsin-digested KS-containing fragments of chick corneal proteoglycans and sequenced the peptides. Results showed that all of the peptides sequenced aligned to the deduced amino acid sequence of either chick lumican or chick keratocan at the first, third, and fourth potential N-linked sites. Sites 1 and 4 in lumican and keratocan are in a homologous location. By analogy with the structure of ribonuclease inhibitor (a Leu-rich repeat containing protein), the KS chains would extend outward on the outer face of a horseshoe-like structure. The amino acid sequences surrounding the potential N-linked sites were also compared. Sites receiving KS tend to have a higher occurrence of aromatic residues, in particular Phe, located within 3 amino acids of NX(S/T). These conserved Phe residues may have a role in the conversion of high mannose N-linke...Continue Reading

References

Jul 1, 1992·Archives of Biochemistry and Biophysics·W LiJ R Hassell
Jun 1, 1990·DNA and Cell Biology·L MadisenA F Purchio
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Jan 1, 1982·Connective Tissue Research·R W FarndaleA J Barrett
Oct 27, 1995·The Journal of Biological Chemistry·E BengtssonY Sommarin
Apr 19, 1996·The Journal of Biological Chemistry·L M CorpuzG W Conrad

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Citations

Apr 8, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J R DunlevyJ R Hassell
Sep 14, 2007·Histochemistry and Cell Biology·E Claire GealyJames R Ralphs
Dec 5, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·L M CorpuzG W Conrad
May 4, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·A J HayesJ R Ralphs
Aug 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Abigail H Conrad, Gary W Conrad
Oct 12, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·L I Melching, P J Roughley
Oct 9, 2002·Pathology International·Yue Ping LuGoro Asano
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·D F HolmesK E Kadler
Jan 7, 2003·IUBMB Life·James L Funderburgh
Aug 11, 2012·Investigative Ophthalmology & Visual Science·Stacy L LittlechildGary W Conrad
Sep 8, 2010·European Journal of Histochemistry : EJH·N KigaS Fujita
May 12, 2001·Japanese Circulation Journal·H BabaG Asano
Nov 24, 1999·DNA Sequence : the Journal of DNA Sequencing and Mapping·E S TashevaG W Conrad
Aug 27, 2015·Biomolecules·Kristian Prydz
Mar 15, 2005·Connective Tissue Research·Gustavo BenevidesLaurecir Gomes
Jul 23, 1999·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M Kurpakus WheaterL D Hazlett
Jul 6, 2010·Experimental Eye Research·John R Hassell, David E Birk
Oct 25, 2008·IUBMB Life·Dragana NikitovicGeorge N Tzanakakis
Jun 4, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Andrew J Quantock, Robert D Young
Sep 19, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Yeqing GengPeter J Roughley
Mar 31, 2015·Experimental Eye Research·Shoujun ChenDavid E Birk
Feb 22, 2002·The Journal of Pathology·Yue Ping LuGoro Asano
Mar 3, 2018·Pharmaceuticals·Vitor H Pomin, Barbara Mulloy
Jan 18, 2018·Glycobiology·Bruce Caterson, James Melrose
Sep 20, 2005·The Journal of Biological Chemistry·Kurt MusselmannJohn R Hassell
Oct 4, 2000·The Journal of Biological Chemistry·J GroverP J Roughley
Jul 7, 2020·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jinyang Zeng-BrouwersLiliana Schaefer
Sep 10, 2020·Frontiers in Cell and Developmental Biology·Sudan PuriVivien J Coulson-Thomas

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