Identification of the non-structural influenza A viral protein NS1A as a bona fide target of the Small Ubiquitin-like MOdifier by the use of dicistronic expression constructs.

Journal of Virological Methods
Sangita PalGermán Rosas-Acosta

Abstract

The cellular SUMOylation system affects the function of numerous viral proteins. Hence, the identification of novel viral targets for the Small Ubiquitin-like MOdifier (SUMO) is key to our understanding of virus-host interactions. The data obtained in this study demonstrate that the non-structural influenza A viral protein NS1A is an authentic SUMO target through the use of a dicistronic expression plasmid containing SUMO (the modifier) and Ubc9 (the SUMO-conjugating enzyme) separated by an Internal Ribosomal Entry Site (IRES). This dual expression plasmid produces a robust increase in cellular SUMOylation, therefore facilitating the characterization of cellular and viral SUMO targets. The identification of NS1A as a bona fide SUMO target suggests, for the first time, a role for SUMOylation during influenza virus infection.

References

Aug 1, 1982·The Journal of General Virology·T PetriN J Dimmock
Dec 31, 1997·FEBS Letters·J M DesterroR T Hay
Jan 28, 1999·Biochemical and Biophysical Research Communications·T OkumaH Yasuda
Apr 3, 1999·The Journal of Biological Chemistry·J M DesterroR T Hay
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·T V PestovaC U Hellen
Jan 17, 2002·Cell·Andrea PichlerFrauke Melchior
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wan-Xiang LiShou-Wei Ding
Jun 11, 2004·Annual Review of Biochemistry·Erica S Johnson
Dec 2, 2004·Biochimica Et Biophysica Acta·R Jürgen Dohmen
Dec 4, 2004·Molecular & Cellular Proteomics : MCP·Germán Rosas-AcostaVan G Wilson
Jun 1, 2005·Molecular and Cellular Biology·Taihei YamaguchiMichael R Kuehn
Apr 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ji-Young Min, Robert M Krug
Jul 4, 2006·Current Opinion in Microbiology·Roberto Boggio, Susanna Chiocca
Aug 22, 2007·Nucleic Acids Research·Astrid JakobsRainer Niedenthal
Nov 15, 2007·Nature Reviews. Molecular Cell Biology·Ruth Geiss-Friedlander, Frauke Melchior
Sep 18, 2008·The Journal of General Virology·Benjamin G HaleDavid Jackson
Mar 7, 2009·The Journal of General Virology·Shohreh MahmoudianManfred Marschall
May 21, 2009·Cell Host & Microbe·Michaela Ulrike GackAdolfo García-Sastre
Sep 5, 2009·Nature Protocols·Michael H TathamRonald T Hay

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Citations

Nov 5, 2010·Journal of Virology·Ke XuBing Sun
Nov 11, 2011·Journal of Virology·Peter WimmerThomas Dobner
Oct 4, 2013·BMC Systems Biology·Yukiko MatsuokaYoshihiro Kawaoka
Dec 3, 2014·Protein Expression and Purification·Gustavo A AvilaChuan Xiao
Aug 12, 2010·Current Opinion in Microbiology·Kasinath ViswanathanVictor DeFilippis
Aug 24, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Murilo T D Bueno, Stéphane Richard
Jun 6, 2015·PLoS Pathogens·Jason E ShoemakerYoshihiro Kawaoka
Apr 5, 2012·Biomolecules·Van G Wilson

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