Identification of the novel lincosamide resistance gene lnu(E) truncated by ISEnfa5-cfr-ISEnfa5 insertion in Streptococcus suis: de novo synthesis and confirmation of functional activity in Staphylococcus aureus

Antimicrobial Agents and Chemotherapy
Qin ZhaoYang Wang

Abstract

The novel lincosamide resistance gene lnu(E), truncated by insertion of an ISEnfa5-cfr-ISEnfa5 segment, was identified in Streptococcus suis. The gene lnu(E) encodes a 173-amino-acid protein with ≤69.4% identity to other lincosamide nucleotidyltransferases. The lnu(E) gene and its promoter region were de novo synthesized, and Staphylococcus aureus RN4220 carrying a shuttle vector with the cloned lnu(E) gene showed a 16-fold increase in the lincomycin MIC. Mass spectrometry experiments demonstrated that Lnu(E) catalyzed the nucleotidylation of lincomycin.

References

Jan 18, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Roland Leclercq
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Adeline AchardRoland Leclercq
Dec 19, 2007·Antimicrobial Agents and Chemotherapy·Efthymia PetinakiRoland Leclercq
Jun 5, 2013·Antimicrobial Agents and Chemotherapy·Yang WangJianzhong Shen

❮ Previous
Next ❯

Citations

Sep 28, 2014·The Journal of Antimicrobial Chemotherapy·Ana C GalesRodrigo E Mendes
Feb 2, 2018·Frontiers in Microbiology·Juan J González-PlazaNikolina Udiković-Kolić
Jun 25, 2014·Antimicrobial Agents and Chemotherapy·A MontillaM Mollerach
Jul 28, 2019·Antimicrobial Resistance and Infection Control·Kaixin ZhouJingyong Sun
Jan 1, 2017·The Journal of Antimicrobial Chemotherapy·Xiao-Qing ZhuJian-Zhong Shen
Sep 29, 2018·Indian Journal of Microbiology·Yosainix C Gaerste-DíazRosa Del C Rocha-Gracia
Nov 3, 2016·Cold Spring Harbor Perspectives in Medicine·Stefan SchwarzBirte Vester
Jul 12, 2018·Microbiology Spectrum·Stefan SchwarzJianzhong Shen
Jun 3, 2021·Clinical Microbiology Reviews·Stefan SchwarzYang Wang
Mar 31, 2018·Microbiology Spectrum·Marisa HaenniJean-Yves Madec

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.