PMID: 9555878Apr 29, 1998Paper

Identification of the omega4400 regulatory region, a developmental promoter of Myxococcus xanthus

Journal of Bacteriology
J P Brandner, Lee Kroos

Abstract

Omega4400 is the site of a Tn5 lac insertion in the Myxococcus xanthus genome that fuses lacZ expression to a developmentally regulated promoter. Cell-cell interactions that occur during development, including C signaling, are required for normal expression of Tn5 lac omega4400. The DNA upstream of the omega4400 insertion has been cloned, the promoter has been localized, and a partial open reading frame has been identified. From the deduced amino acid sequence of the partial open reading frame, the gene disrupted by Tn5 lac omega4400 may encode a protein with an ATP- or GTP-binding site. Expression of the gene begins 6 to 12 h after starvation initiates development, as measured by beta-galactosidase production in cells containing Tn5 lac omega4400. The putative transcriptional start site was mapped, and deletion analysis has shown that DNA downstream of -101 bp is sufficient for C-signal-dependent, developmental activation of this promoter. A deletion to -76 bp eliminated promoter activity, suggesting the involvement of an upstream activator protein. The promoter may be transcribed by RNA polymerase containing a novel sigma factor, since a mutation in the M. xanthus sigB or sigC gene did not affect Tn5 lac omega4400 expression ...Continue Reading

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Citations

Nov 14, 2000·Journal of Bacteriology·A G GarzaM Singer
Jan 25, 2011·Journal of Bacteriology·Jun-Seok LeeLee Kroos
Apr 20, 2006·Journal of Bacteriology·Poorna ViswanathanLee Kroos

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