PMID: 9434154Jan 20, 1998Paper

Identification of the open reading frame for the Pseudomonas putida D-hydantoinase gene and expression of the gene in Escherichia coli

Biochimica Et Biophysica Acta
H R ChienW H Hsu

Abstract

A DNA fragment containing the gene for D-hydantoinase was cloned from Pseudomonas putida CCRC 12857 into Escherichia coli. The cloned gene contained an open reading frame (ORF) of 1485 nucleotides encoding a protein of 53.4 kDa in which the carboxyl terminal end is longer than that previously deduced from strain DSM 84. This ORF was verified by amino acid sequencing of amino and carboxyl termini, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and amino acid sequence comparison. Deletion analysis revealed that 32 amino acids from the carboxyl terminal end were essential for D-hydantoinase activity. Tagging of six consecutive histidyl residues to the amino terminus or to the carboxyl terminus of the enzyme did not significantly affect D-hydantoinase activity. Under the control of T5lac promoter and lactose induction, the D-hydantoinase activity of transformed E. coli reached 200 U l-1 which is about 20-fold higher than that of gene donor strain.

References

Jun 1, 1976·Bacteriological Reviews·G D Vogels, C Van der Drift
Jan 1, 1977·Methods in Enzymology·R Hayashi
Jan 1, 1990·Applied and Environmental Microbiology·J J ChalmersD B Wilson
Oct 1, 1995·The Annals of Thoracic Surgery·G MassardG Morand
Jun 7, 1996·Biochimica Et Biophysica Acta·K MatsudaN Tamaki
Jan 1, 1972·Methods in Enzymology·R P Ambler

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Citations

Apr 6, 2004·Applied Microbiology and Biotechnology·G F MatcherR A Dorrington
Dec 22, 1999·Current Opinion in Biotechnology·B Schulze, M G Wubbolts
May 2, 2000·Applied and Environmental Microbiology·G J KimH S Kim
Feb 26, 2016·Environmental Microbiology·Eugeni BeldaClaudine Médigue

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