Identification of the potential target genes of microRNA-146a induced by PMA treatment in human microvascular endothelial cells

Experimental Cell Research
Ching-Hua HsiehWen-Neng Chang

Abstract

Phorbol 12-myristate 13-acetate (PMA) is known to activate protein kinase C (PKC) and increase angiogenesis in cultured endothelial cells. Using a microRNA (miRNA) array, we found that PMA induced miR-146a expression in human microvascular endothelial cells. The miR-146a expression was dependent on dose and time and independent of PKC activation. Using a combined approach involving predictions using miRanda algorithm and whole genome microarray experiments with or without inhibition of miR-146a expression by LNA-antimir-146a or LNA-control, 29 potential target genes of miR-146a were identified. Because endothelial cell S phase progression is an early event in the induction of angiogenesis, we evaluated 5 cell cycle-related genes from the 29 target genes and found that the transcripts of 3 genes (CCNA2, PA2G4, and BRCA1) were downregulated after PMA treatment, but their expression was rescued upon miR-146a inhibition. However, inhibition of miR-146a expression failed to alter the cell cycle distribution or angiogenesis induced by PMA treatment. By using a combined approach involving computational prediction and a whole genome microarray experiment in the presence or absence of antimir, the observations in this presented article ...Continue Reading

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Citations

May 28, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Cheng-Shyuan RauChing-Hua Hsieh
Nov 6, 2013·Disease Markers·S VimalrajN Selvamurugan
May 4, 2011·Journal of Cellular Biochemistry·Jing WangYuxiang Zhou
Dec 19, 2012·Biochemical and Biophysical Research Communications·Huang-Joe WangLi-Jen Lin
Dec 11, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S TimmermansR E Vandenbroucke
Jun 25, 2016·International Journal of Molecular Sciences·Lei WangHong Zhang
Jun 18, 2010·BMC Musculoskeletal Disorders·Ching-Hua HsiehChia-Jung Lin
Mar 26, 2016·Oncotarget·Catherine MénardPrzemyslaw Sapieha

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