Identification of the principal transcriptional regulators for low-fat and high-fat meal responsive genes in small intestine

Nutrition & Metabolism
Octave MucunguziJonny St-Amand

Abstract

High-fat (HF) diet is a well-known cause of obesity. To identify principle transcriptional regulators that could be therapeutic targets of obesity, we investigated transcriptomic modulation in the duodenal mucosa following low-fat (LF) and HF meal ingestion. Whereas one group of mice was sacrificed after fasting, the others were fed ad libitum with LF or HF meal, and sacrificed 30 min, 1 h and 3 h after the beginning of the meal. A transcriptome analysis of the duodenal mucosa of the 7 groups was conducted using both microarray and serial analysis of gene expression (SAGE) method followed by an Ingenuity Pathways Analysis (IPA). SAGE and microarray showed that the modulation of a total of 896 transcripts in the duodenal mucosa after LF and/or HF meal, compared to the fasting condition. The IPA identified lipid metabolism, molecular transport, and small molecule biochemistry as top three molecular and cellular functions for the HF-responsive, HF-specific, HF-delay, and LF-HF different genes. Moreover, the top transcriptional regulator for the HF-responsive and HF-specific genes was peroxisome proliferator-activated receptor alpha (PPARα). On the other hand, the LF-responsive and LF-specific genes were related to carbohydrate met...Continue Reading

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Citations

Jan 17, 2021·Medicina·Abdelaziz GhanemiJonny St-Amand
Jan 27, 2021·Animals : an Open Access Journal From MDPI·Abdelaziz GhanemiJonny St-Amand
Oct 8, 2019·The International Journal of Biochemistry & Cell Biology·Abdelaziz GhanemiJonny St-Amand
May 1, 2021·Medicina·Abdelaziz GhanemiJonny St-Amand

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Methods Mentioned

BETA
electrophoresis
PCR
protein folding

Software Mentioned

affylmGUI Graphical User Interface
ImageJ
SAGE
Ingenuity Pathways Analysis ( IPA )
IPA
limma microarray package

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