Abstract
Axl, Sky, and Mer, members of an Axl/Sky receptor tyrosine kinase subfamily, are typified by the cell adhesion molecule-related extracellular domain. The product of growth arrest-specific gene 6 (Gas6), structurally homologous to the anticoagulant protein S, was recently identified as the ligand for Axl and Sky, but the ligand for Mer remained unknown. We have now obtained evidence that Gas6 can also function as a ligand for Mer. Co-precipitation analysis, using soluble receptors of Axl, Sky, and Mer (Axl-Fc, Sky-Fc, and Mer-Fc) composed of the extracellular domain of receptors fused to the Fc domain of immunoglobulin G1, clearly showed that Gas6, but not protein S, specifically bound to Axl-Fc, Sky-Fc, and Mer-Fc fusion proteins. Quantitative kinetic analyses using a BIAcore biosensor instrument revealed dissociation constants (Kd) of the binding of rat Gas6 to Axl-Fc, Sky-Fc, and Mer-Fc are 0.4, 2.7, and 29 nM, respectively. We also found that Gas6 stimulated tyrosine phosphorylation of Axl, Sky, and Mer receptors ectopically expressed in Chinese hamster ovary cells. Taken together, these findings suggest that Gas6 is a common ligand for Axl, Sky, and Mer, all known members of an Axl/Sky receptor subfamily.
References
Oct 1, 1992·Journal of Virology·R JiaH Hanafusa
Jul 26, 1991·Science·E H FischerN K Tonks
Oct 1, 1991·Molecular and Cellular Biology·J P O'BryanE T Liu
Dec 15, 1991·Journal of Immunological Methods·R KarlssonL Mattsson
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
May 15, 1987·Science·B A CunninghamG M Edelman
Sep 1, 1983·Journal of Biochemistry·K SuzukiS Hashimoto
Sep 7, 1995·Annals of the New York Academy of Sciences·M Barbacid
Jun 1, 1995·Journal of Biochemistry·K OhashiK Mizuno
Dec 1, 1995·Nature Genetics·T TsukamotoT Osumi
Apr 17, 1995·Biochemical and Biophysical Research Communications·J ToshimaK Mizuno
Sep 29, 1995·The Journal of Biological Chemistry·K OhashiK Mizuno
Aug 11, 1995·Cell·P J GodowskiR G Hammonds
Jan 1, 1993·Annual Review of Biochemistry·W J FantlL T Williams
Feb 16, 1995·Nature·B C VarnumR J Toso
Feb 24, 1995·Cell·T N StittP F Jones
Mar 17, 1995·The Journal of Biological Chemistry·T NakanoH Arita
Mar 24, 1995·The Journal of Biological Chemistry·I C TaylorH E Varmus
Aug 1, 1993·Molecular and Cellular Biology·G ManfiolettiC Schneider
Dec 1, 1995·Molecular and Cellular Biology·L Ling, H J Kung
Dec 29, 1995·The Journal of Biological Chemistry·I OkanoK Mizuno
Dec 29, 1995·The Journal of Biological Chemistry·J ToshimaK Mizuno
Feb 1, 1996·Current Opinion in Neurobiology·G C Friedman, D D O'Leary
Citations
Jul 3, 1999·Cancer·A WimmelM Schuermann
Apr 12, 2002·Journal of Neuroscience Research·Hiroshi FunakoshiToshikazu Nakamura
Jul 26, 2002·International Journal of Hematology·F Jon GeskeValerie A Fadok
Sep 3, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Faraaz B Chekeni, Kodi S Ravichandran
Jul 6, 2012·Clinical Rheumatology·Chien-Sheng WuPing-Ning Hsu
Feb 9, 2010·Apoptosis : an International Journal on Programmed Cell Death·Adrian E Morelli, Adriana T Larregina
Aug 24, 2012·Journal of Clinical Immunology·Hyoun-Ah KimChang-Hee Suh
Feb 6, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Christian GrommesGary E Landreth
Jun 14, 2005·Experimental Eye Research·Michael O HallToshka A Abrams
Nov 20, 2003·Trends in Cell Biology·Cynthia Grimsley, Kodi S Ravichandran
Mar 30, 2004·Trends in Endocrinology and Metabolism : TEM·Margaret E WiermanSheila Nielsen-Preiss
Jan 16, 2003·Neuropharmacology·T YagamiH Tsuzuki
Jul 6, 2000·Experimental Hematology·C DarbyS G Emerson
Apr 19, 2002·Biochimica Et Biophysica Acta·Leon J Schurgers, Cees Vermeer
Dec 24, 2002·Current Opinion in Immunology·Greg Lemke, Qingxian Lu
Jan 29, 2013·Blood Cancer Journal·L N BrandaoD K Graham
Nov 27, 2007·Nature Reviews. Immunology·Kodi S Ravichandran, Ulrike Lorenz
Apr 19, 2008·Nature Reviews. Immunology·Greg Lemke, Carla V Rothlin
Sep 24, 2013·Oncogene·M A CichońE A O'Toole
Aug 3, 2002·European Journal of Biochemistry·Connie C S Wong, Will M Lee
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Shoutian ZhuXu Wu
Aug 11, 2011·The Journal of Biological Chemistry·Edward ThorpIra Tabas
Oct 17, 2012·The Journal of Experimental Medicine·Thomas BauerHerbert Strobl
Apr 16, 2002·DNA and Cell Biology·Rosalia C M SimmenFrank A Simmen
Dec 20, 2002·Thyroid : Official Journal of the American Thyroid Association·Masahiro ItoShunichi Yamashita
May 3, 2000·Journal of Hematotherapy & Stem Cell Research·S Sinha, S J Corey
Mar 2, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Yan ZhangXiaojing Ma
Jul 20, 1999·Thyroid : Official Journal of the American Thyroid Association·T ItoI Sekine
Jun 29, 2012·Molecular Biology of the Cell·Hyun-Jung ParkJihee Lee Kang
Sep 22, 2012·Cardiovascular Research·Ulisse GarbinAnna Fratta Pasini
Aug 9, 2012·The Journal of Antimicrobial Chemotherapy·Shaswat KansalPrabhat Ranjan Mishra
Jun 17, 2004·Current Opinion in Nephrology and Hypertension·Motoko Yanagita
Jan 14, 2012·Current Opinion in Nephrology and Hypertension·Sri N Batchu, Vyacheslav A Korshunov
Aug 1, 2009·Current Opinion in Lipidology·Marc TjwaEsther Lutgens
May 15, 1997·European Journal of Biochemistry·P NybergP García de Frutos
Oct 14, 2004·Journal of Virology·Yuling LiShannon Kenney
Jan 5, 2002·Molecular and Cellular Biology·Melissa P AllenMargaret E Wierman
Oct 18, 2005·Molecular and Cellular Biology·Vadim BudagianSilvia Bulfone-Paus
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Peter W Reddien, H Robert Horvitz
Feb 22, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Mony ShuvyChaim Lotan
Feb 6, 2010·Physiology·Brian M Kevany, Krzysztof Palczewski
Sep 23, 2010·Cancer Research·Erinn B RankinAmato J Giaccia
Apr 21, 2012·Molecular Cancer Research : MCR·Anjali MishraRussell S Taichman
May 3, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Edward ThorpIra Tabas
Oct 7, 2003·American Journal of Respiratory Cell and Molecular Biology·Bin HuJeffrey L Curtis
Jan 12, 2008·The Journal of Clinical Investigation·Anne Angelillo-ScherrerPeter Carmeliet
Jul 23, 2013·The Journal of Clinical Investigation·Rebecca S CookH Shelton Earp
Oct 19, 2006·Blood·Susan SatherDouglas K Graham
Oct 9, 2008·Blood·Il-Kyoo ParkMichael A Caligiuri
Aug 12, 2009·Blood·Zuoan YiRoland Tisch