Identification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens

Endocrinology
Yajun WangFrederick C Leung

Abstract

Prolactin-releasing peptide (PrRP) and its structurally related peptide, Carassius Arg-Phe-amide peptide (C-RFa), have been reported to play similar roles in regulating food intake and pituitary functions in vertebrates. However, the identity, functionality, and expression of the receptor(s) for PrRP and C-RFa remain largely unknown in nonmammalian vertebrates, including birds. In this study, three receptors homologous to mammalian PrRP receptor (PrRPR), named cPrRPR1, cPrRPR2, and cC-RFaR, respectively, were cloned from chicken brain by RT-PCR. Using a pGL3-NFAT-RE-luciferase reporter system, we demonstrated that cPrRPR1 and cPrRPR2 expressed in Chinese hamster ovarian cells could be activated by cPrRP₂₀ and cC-RFa₂₀ potently, whereas cC-RFaR could only be activated effectively by cC-RFa₂₀ (EC₅₀, 0.11 nM), indicating that cPrRPR1 and cPrRPR2 can function as common receptors for PrRP and C-RFa, whereas cC-RFaR is a receptor specific to C-RFa. Using a pGL3-CRE-luciferase reporter system, cPrRPR1, cPrRPR2, and cC-RFaR expressed in Chinese hamster ovarian cells were also shown to activate intracellular protein kinase A signaling pathway upon cC-RFa₂₀ treatment (100 nM). Moreover, RT-PCR assay revealed that cPrRPR1, cPrRPR2, and cC...Continue Reading

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Citations

Aug 14, 2013·Journal of Molecular Endocrinology·Guixian BuYajun Wang
Dec 30, 2014·Frontiers in Endocrinology·Guro Katrine SandvikFinn-Arne Weltzien
Aug 29, 2014·Frontiers in Endocrinology·Seongsik YunJae Young Seong
Nov 27, 2014·Frontiers in Endocrinology·Tetsuya Tachibana, Tatsuya Sakamoto
May 14, 2017·Journal of Molecular Endocrinology·Chunheng MoYajun Wang
May 9, 2019·Scientific Reports·Maiju RinneHenri Xhaard
Oct 28, 2019·International Journal of Molecular Sciences·Veronika PražienkováLenka Maletínská
Nov 18, 2016·Frontiers in Neuroscience·Tetsuya Tachibana, Kazuyoshi Tsutsui
May 1, 2021·International Journal of Molecular Sciences·Yulong SunYuanyuan Kuang
Nov 23, 2021·Frontiers in Endocrinology·Chuanhui XiaGuangfu Hu

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