Identification of the replication terminator protein binding sites in the terminus region of the Bacillus subtilis chromosome and stoichiometry of the binding

Journal of Molecular Biology
P J LewisR G Wake

Abstract

DNase I footprinting of the interaction between the replication terminator protein (RTP) of Bacillus subtilis and the inverted repeat region (IRR) at the chromosome terminus, to which it binds to block the clockwise replication fork, showed that two major regions of 41 base pairs (bp) were protected from cleavage. These regions corresponded approximately to the imperfect inverted repeats (IRI and IRII) identified previously. Band retardation analyses of the interaction between RTP and portions of the IRR established that each inverted repeat (IRI or IRII) contained two RTP binding sites. By sedimentation equilibrium in the ultracentrifuge, RTP was found to exist as a dimer of 29 kDa at neutral pH and concentrations above 0.2 g/l. Quantitative studies of the RTP-IRR interaction using [3H]RTP and [32P]IRR showed that the fully saturated complex contained eight RTP monomers per IRR. It is concluded that a dimer of RTP binds to each of the four sites in IRR. The apparent dissociation constant for the interaction was estimated (in the presence of 50% glycerol) to be 1.2 x 10(-11) M (dimer of RTP). Glycerol was found to have a marked effect on the affinity of RTP for the IRR and on the relative amounts of the interaction complexes fo...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A D JohnsonM Ptashne
Jun 16, 1989·Cell·S HandeliH Cedar
Dec 1, 1984·Journal of Biochemical and Biophysical Methods·W Ansorge, S Labeit
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D BastiaJ Ram

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Citations

Sep 1, 1991·Research in Microbiology·P J Lewis, R G Wake
Jan 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·K P LemonA D Grossman
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·S KaulD Bastia
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·K S PaiD Bastia
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·A C MannaD Bastia
Nov 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·J P VivianM C J Wilce
Aug 31, 2006·Molecular and Cellular Biology·Gregor Krings, Deepak Bastia
May 12, 2016·Nucleic Acids Research·M Charl MoolmanNynke H Dekker
Jan 12, 2017·Genes·Katie H Jameson, Anthony J Wilkinson
May 28, 2014·FEMS Microbiology Letters·Valentin V Rybenkov

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