Identification of the retinoic acid-inducible all-trans-retinoic acid 4-hydroxylase.

The Journal of Biological Chemistry
J A WhiteM Petkovich

Abstract

Retinoic acid (RA) metabolites of vitamin A are key regulators of gene expression involved in embryonic development and maintenance of epithelial tissues. The cellular effects of RA are dependent upon the complement of nuclear receptors expressed (RARs and RXRs), which transduce retinoid signals into transcriptional regulation, the presence of cellular retinoid-binding proteins (CRABP and CRBP), which may be involved in RA metabolism, and the activity of RA metabolizing enzymes. We have been using the zebrafish as a model to study these processes. To identify genes regulated by RA during exogenous RA exposure, we utilized mRNA differential display. We describe the isolation and characterization of a cDNA, P450RAI, encoding a novel member of the cytochrome P450 family. mRNA transcripts for P450RAI are expressed normally during gastrulation, and in a defined pattern in epithelial cells of the regenerating caudal fin in response to exogenous RA. In COS-1 cells transfected with the P450RAI cDNA, all-trans-RA is rapidly metabolized to more polar metabolites. We have identified 4-oxo-RA and 4-OH-RA as major metabolic products of this enzyme. P450RAI represents the first enzymatic component of RA metabolism to be isolated and characte...Continue Reading

References

Jul 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Maden, N Holder
May 15, 1987·Archives of Biochemistry and Biophysics·J L Napoli, K R Race
Apr 15, 1987·Biochemical Pharmacology·J B Williams, J L Napoli
Jan 27, 1995·The Journal of Biological Chemistry·C ZieroldH F DeLuca
Dec 15, 1995·Cell·D J Mangelsdorf, R M Evans
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·B BlumbergR M Evans

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Citations

Oct 24, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·I KostetskiiM Zile
Jul 14, 1999·Developmental Genetics·L DeltourG Duester
Dec 28, 1999·BioFactors·E C Swindell, G Eichele
Jul 27, 2001·The Journal of Experimental Zoology·E Martinez-Ceballos, C A Burdsal
Nov 5, 1999·Archives of Biochemistry and Biophysics·D R Nelson
Dec 22, 1999·Biochemical and Biophysical Research Communications·T Omura
Oct 12, 2012·Cell and Tissue Research·Jing-jing MaJing-pian Peng
Nov 1, 2003·Archives of Biochemistry and Biophysics·Jason S ChapmanMargaret Clagett-Dame
Jun 1, 2001·Advances in Enzyme Regulation·L LuuM Petkovich
May 9, 2000·Progress in Lipid Research·A Azzi, A Stocker
Jul 9, 2002·Brain Research. Developmental Brain Research·Marina E Trofimova-Griffin, Mont R Juchau
Mar 23, 2000·Brain Research. Developmental Brain Research·M E Trofimova-GriffinM R Juchau
Jun 12, 2003·Molecular and Cellular Neurosciences·Sonia GuidatoSarah Guthrie
May 19, 2000·Cytokine & Growth Factor Reviews·P McCaffery, U C Dräger
Oct 16, 1999·Biochimica Et Biophysica Acta·J L Napoli
Nov 2, 2001·Trends in Molecular Medicine·J Rossant, C McKerlie
May 29, 2000·The Proceedings of the Nutrition Society·M Maden
Nov 2, 2013·Chemical Reviews·Alexander R MoiseEleanore T Wurtzel
Nov 26, 2013·Chemical Reviews·Rosana ÁlvarezÁngel R de Lera
Jun 11, 2008·Nature Reviews. Genetics·Karen Niederreither, Pascal Dollé
Nov 5, 2002·Nature Reviews. Neuroscience·Malcolm Maden
Jun 7, 2000·The Biochemical Journal·N Noy
Oct 14, 2010·Human Reproduction·Hong-Fei XiaJing-Pian Peng
Jul 24, 2010·Biology of Reproduction·Elizabeth M SnyderMichael D Griswold
Aug 21, 2001·Genes & Development·T KoideK Umesono
Apr 28, 2011·Molecular Pharmacology·Jayne E ThatcherNina Isoherranen
Jun 11, 2002·Annual Review of Nutrition·Margaret Clagett-Dame, Hector F DeLuca
May 20, 1999·Annual Review of Pharmacology and Toxicology·M D Collins, G E Mao
Feb 22, 2001·Annual Review of Physiology·W V Cardoso
Apr 1, 2006·American Journal of Respiratory Cell and Molecular Biology·Malcolm Maden
Dec 21, 2007·BMC Developmental Biology·Leigh J WilsonRichard J T Wingate

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