Identification of the Rhodobacter sphaeroides SOS box

Molecular Microbiology
Antonio R Fernández de HenestrosaJordi Barbé

Abstract

Gel-mobility shift assays with crude cell extracts of Rhodobacter sphaeroides, which belongs to the alpha group of the proteobacteria, have shown that a protein binds to the promoter of its recA gene, resulting in two retardation bands. Analysis of the minimal region of the R. sphaeroides recA gene required for the formation of the DNA-protein complexes, revealed the presence of the motifs GTTCN7GATC and GAACN7GAAC, which are centred at positions -21 and +8 from the transcriptional starting point respectively. Using PCR mutagenesis, we have demonstrated that these two motifs are required for the formation of both DNA-protein complexes in vitro as well as for the DNA damage-mediated inducibility of the recA gene in vivo. Furthermore, the level of the recA gene expression in the constitutive mutants is the same as that achieved by the wild-type cells after DNA damage, indicating that the binding protein must be a repressor. The motif GTTCN7GTTC is also present upstream of the R. sphaeroides uvrA promoter, which in vitro specifically binds to a protein and whose expression is DNA damage inducible. Mutagenesis of this motif abolishes both the binding of this protein to the uvrA promoter and the DNA damage-mediated expression of thi...Continue Reading

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Citations

Apr 26, 2008·Molecular Genetics and Genomics : MGG·Mei-Kwei YangVin-Long Sung
Aug 10, 2006·Antonie van Leeuwenhoek·Gerard MazónJordi Barbé
Feb 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yingfang LiuGongyi Zhang
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y LiuG Zhang
May 12, 2005·Nucleic Acids Research·Rodrigo S GalhardoCarlos F M Menck
Jan 13, 2006·Nucleic Acids Research·Ivan ErillJordi Barbé
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·Montserrat SabatéGuillem Prats
Oct 11, 2002·Journal of Bacteriology·Antonio R Fernández de HenestrosaJordi Barbé
Jul 18, 2003·Journal of Bacteriology·Antonio R Fernández de HenestrosaJordi Barbé
Jul 21, 2005·Journal of Bacteriology·Susana CampoyJordi Barbé
Mar 14, 2007·BMC Microbiology·Marinalva Martins-PinheiroCarlos F M Menck
Nov 12, 2003·Emerging Infectious Diseases·Guillem PratsLluís Salleras
Jun 17, 2008·FEBS Letters·Laura M Patterson-Fortin, George W Owttrim
Jun 14, 2014·FEMS Microbiology Reviews·Zeynep Baharoglu, Didier Mazel
Sep 22, 2007·FEMS Microbiology Reviews·Ivan ErillJordi Barbé
Aug 5, 2016·Frontiers in Molecular Biosciences·Ivan ErillJordi Barbé
Jun 16, 2017·Environmental Microbiology·Miquel Sánchez-OsunaIvan Erill
Nov 6, 2004·Microbiology·Gerard MazónJordi Barbé
Feb 13, 1999·FEMS Microbiology Letters·M LabaziJ Barbé
Apr 12, 2000·Molecular Microbiology·A R Fernández De HenestrosaR Woodgate

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