Feb 1, 1992

Identification of the sea urchin egg receptor for sperm using an antiserum raised against a fragment of its extracellular domain

The Journal of Cell Biology
K R Foltz, W J Lennarz

Abstract

Sea urchin egg fertilization requires the species-specific interaction of molecules on the sperm and egg surfaces. Previously, we isolated an extracellular, 70-kD glycosylated fragment of the S. purpuratus egg receptor for sperm by treating the eggs with lysylendoproteinase C (Foltz, K. R., and W. J. Lennarz. 1990. J. Cell Biol. 111:2951-2959). To characterize the receptor further, we have generated a polyclonal antiserum (anti-70KL) against the purified 70-kD fragment. Anti-70KL was found to react with a single polypeptide of approximately 350 kD on Western blots, presumed to be the intact receptor, in an egg cell surface preparation. This polypeptide appeared to be tightly associated with the plasma membrane/vitelline layer complex, as it was released from these preparations only by detergent treatment. Immunofluorescence microscopy revealed that the receptor was distributed evenly over the egg surface. The anti-70KL was species specific both in its ability to recognize the egg surface protein and to inhibit sperm binding. Fab fragments generated from affinity-purified anti-70KL also bound to the egg surface and inhibited sperm binding in a concentration-dependent manner. Interestingly, treatment with Fabs caused a small perc...Continue Reading

  • References34
  • Citations24

References

Mentioned in this Paper

ZP3 gene
Ovum
Extracellular
Hormone Receptors, Cell Surface
Immune Sera
Immunofluorescence Microscopy
Sperm-Ovum Interactions
Fab Immunoglobulins
Sea urchin (invertebrate)
Egg surface sperm receptor

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