PMID: 8589454Nov 1, 1995Paper

Identification of the sequences in HMG-CoA reductase required for karmellae assembly

Molecular Biology of the Cell
M L ParrishR Wright

Abstract

In all eukaryotic cells that have been examined, specific membrane arrays are induced in response to increased levels of the ER membrane protein, HMG-CoA reductase. Analysis of these inducible membranes has the potential to reveal basic insights into general membrane assembly. Yeast express two HMG-CoA reductase isozymes, and each isozyme induces a morphologically distinct proliferation of the endoplasmic reticulum. The isozyme encoded by HMG1 induces karmellae, which are long stacks of membranes that partially enclose the nucleus. In contrast, the isozyme encoded by HMG2 induces short stacks of membrane that may be associated with the nucleus, but are frequently present at the cell periphery. To understand the molecular nature of the different cellular responses to Hmg1p and Hmg2p, we mapped the region of Hmg1p that is needed for karmellae assembly. For this analysis, a series of exchange alleles was examined in which a portion of the Hmg2p membrane domain was replaced with the corresponding Hmg1p sequences. Results of this analysis indicated that the ER lumenal loop between predicted transmembrane domains 6 and 7 was both necessary and sufficient for karmellae assembly, when present in the context of an HMG-CoA reductase memb...Continue Reading

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Citations

May 7, 2008·The Journal of Biological Chemistry·Gil-Soo HanSymeon Siniossoglou
Jan 26, 2002·Molecular Biology of the Cell·Laura Block-AlperDavid I Meyer
Dec 31, 2003·Molecular Biology of the Cell·Javier Peña-DiazDolores Gonzalez-Pacanowska
Aug 1, 2008·Molecular Biology of the Cell·Christine M FederovitchRandolph Y Hampton
Mar 16, 2012·Genetics·Gregory Prelich
Aug 27, 2014·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·William Dauer
Oct 29, 2003·The Journal of Cell Biology·Erik L SnappJennifer Lippincott-Schwartz
Dec 27, 2005·Fungal Genetics and Biology : FG & B·Kyeyong SeongJin-Rong Xu
Jul 17, 2015·PLoS Genetics·Zhanna Lipatova, Nava Segev
May 29, 2003·The Journal of Biological Chemistry·Yoshinori MurataHitoshi Iwahashi
Nov 17, 2004·The Journal of Biological Chemistry·Roy AmarilioSima Lev
Feb 24, 2001·Traffic·K S Powell, M Latterich
Nov 18, 2004·Journal of Cell Science·Thorsten RalleReimer Stick

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