Identification of the Sfp-Type PPTase EppA from the Lichenized Fungus Evernia prunastri

PloS One
Olivia SchimmingHelge B Bode

Abstract

In the last decades, natural products from lichens have gained more interest for pharmaceutical application due to the broad range of their biological activity. However, isolation of the compounds of interest directly from the lichen is neither feasible nor sustainable due to slow growth of many lichens. In order to develop a pipeline for heterologous expression of lichen biosynthesis gene clusters and thus the sustainable production of their bioactive compounds we have identified and characterized the phosphopantheteinyl transferase (PPTase) EppA from the lichen Evernia prunastri. The Sfp-type PPTase EppA was functionally characterized through heterologous expression in E. coli using the production of the blue pigment indigoidine as readout and by complementation of a lys5 deletion in S. cerevisiae.

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Datasets Mentioned

BETA
KT369532

Methods Mentioned

BETA
PCR

Software Mentioned

ClustalW
Geneious
PhyML
BLAST

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