Identification of the sigmaE regulon of Salmonella enterica serovar Typhimurium

Microbiology
Henrieta SkovierovaJan Kormanec

Abstract

The extracytoplasmic function sigma factor, sigma(E), has been shown to play a critical role in virulence of Salmonella enterica serovar Typhimurium (S. Typhimurium). The previously optimized two-plasmid system has been used to identify S. Typhimurium promoters recognized by RNA polymerase containing sigma(E). This method allowed identification of 34 sigma(E)-dependent promoters that direct expression of 62 genes in S. Typhimurium, 23 of which (including several specific for S. Typhimurium) have not been identified previously to be dependent upon sigma(E) in Escherichia coli. The promoters were confirmed in S. Typhimurium and transcriptional start points of the promoters were determined by S1-nuclease mapping. All the promoters contained sequences highly similar to the consensus sequence of sigma(E)-dependent promoters. The identified genes belonging to the S. Typhimurium sigma(E)-regulon encode proteins involved in primary metabolism, DNA repair systems and outer-membrane biogenesis, and regulatory proteins, periplasmic proteases and folding factors, proposed lipoproteins, and inner- and outer-membrane proteins with unknown functions. Several of these sigma(E)-dependent genes have been shown to play a role in virulence of S. T...Continue Reading

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Citations

Aug 11, 2011·Journal of Theoretical Biology·Scheila de Avila E SilvaGünther J L Gerhardt
Feb 17, 2015·Journal of Proteome Research·Jie LiJoshua N Adkins
Dec 14, 2006·Molecular Microbiology·Alexandra SittkaJörg Vogel
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Jul 18, 2006·FEMS Microbiology Letters·Henrieta SkovierovaJan Kormanec
Feb 10, 2016·Scientific Reports·Elin VerbruggheFrank Pasmans
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May 26, 2018·Critical Reviews in Biochemistry and Molecular Biology·Hong-Leong CheahThean-Hock Tang
Oct 6, 2017·FEMS Microbiology Reviews·Sylvie ChevalierPierre Cornelis

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