PMID: 8971927Jan 1, 1996Paper

Identification of the site of glycation of human insulin

Peptides
F P O'HarteP R Flatt

Abstract

This study evaluates the nature of glycated human insulin formed following exposure to hyperglycemic conditions in vitro. Glycated insulin was purified by RP-HPLC and its molecular mass (5971.3 Da) determined by plasma desorption mass spectrometry (MS). The difference in mass (163.7 Da) from nonglycated insulin (5807.6 Da) corresponds to a single reduced glucose (glucitol) residue. Following reduction of insulin disulfide bridges, MS confirmed that the B-chain was glycated. Enzymatic digestions with trypsin, endoproteinase Glu-C, and thermolysin, followed by RP-HPLC and identification of fragments by MS, localized glycation to the B-chain (1-5) region. Electrospray tandem MS identified the site of glycation as the B-chain NH2-terminal Phe1 residue. This was confirmed by automated Edman degradation with glycated human insulin.

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Citations

May 25, 2010·Analytical and Bioanalytical Chemistry·Sofia GuedesPedro Domingues
Feb 22, 2012·Zeitschrift für Gerontologie und Geriatrie·A SimmN Nass
Apr 16, 2013·Applied Biochemistry and Biotechnology·Parnian AlaviAli Akbar Moosavi-Movahedi
Apr 17, 2009·Journal of the American Society for Mass Spectrometry·Sofia GuedesPedro Domingues
Jun 6, 2009·Antioxidants & Redox Signaling·Anne Negre-SalvayreManuel Portero-Otín
Aug 21, 2001·Biochemical and Biophysical Research Communications·A M McKillopF P O'Harte
Jun 26, 2009·Cellular and Molecular Life Sciences : CMLS·Robert Root-Bernstein, Jessica Vonck
Mar 19, 2015·Biomolecules·Kerstin NowotnyTilman Grune
Nov 1, 2015·Biochimica Et Biophysica Acta·Clara IannuzziIvana Sirangelo
Sep 17, 2005·Biochimica Et Biophysica Acta·M Abul FarahYangsun Kim
May 22, 2003·Metabolism: Clinical and Experimental·John R LindsayFinbarr P M O'harte
Jul 21, 2016·Glycoconjugate Journal·Naila RabbaniPaul J Thornalley
Oct 28, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Maryam ChinisazAli Akbar Moosavi-Movahedi
Apr 4, 2007·European Journal of Mass Spectrometry·Celina SantosJorge Caldeira
Mar 21, 2020·Chemistry : a European Journal·Mads ØstergaardKnud J Jensen
Jun 12, 2019·Nature Communications·Landa PurushottamVishal Rai
Mar 1, 1997·The Proceedings of the Nutrition Society·P R FlattF P O'Harte
Jan 29, 2021·Journal of Biochemical and Molecular Toxicology·C R Sruthi, K G Raghu
Feb 15, 2018·Archives of Biochemistry and Biophysics·Leila ShahriyaryAli Akbar Saboury
Jul 3, 2021·International Journal of Molecular Sciences·Ivana Sirangelo, Clara Iannuzzi
Aug 24, 2019·The Journal of Physical Chemistry. B·Li Hung C ChungOlaf J Rolinski

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