Identification of the spermatogenic zip protein Spz1 as a putative protein phosphatase-1 (PP1) regulatory protein that specifically binds the PP1cgamma2 splice variant in mouse testis

The Journal of Biological Chemistry
C Hrabchak, Susannah Varmuza

Abstract

The spermatogenic zip protein (Spz1) was originally isolated from a mouse testis library and identified as a novel member of the basic helix-loop-helix family of transcription factors. Here we identify Spz1 as a specific binding partner of the gamma2 catalytic subunit of protein phosphatase-1. Male mice homozygous for a null mutation in the protein phosphatase-1cgamma (PP1cgamma) gene are infertile and display a distinct impairment in spermiogenesis despite the continued presence of closely related PP1c isoforms. Yeast two-hybrid screening using the PP1cgamma2 splice variant has identified Spz1 as an interacting protein and possible mediator of the sterile PP1cgamma mutant phenotype. Spz1 was shown to interact specifically with PP1cgamma2 but did not show an interaction with PP1calpha or with a truncated version of PP1cgamma2 lacking 18 amino acids from the C terminus. Interaction between full-length Spz1 and PP1cgamma2 was verified by co-immunoprecipitation and co-localization experiments in COS-1 cells as well as gel-shift and sedimentation assays using whole testis lysates. Immunohistochemistry on wild type testis sections reveals a stage-specific expression pattern for Spz1 during spermatogenesis that appeared grossly abnor...Continue Reading

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Citations

Jan 2, 2007·Cloning and Stem Cells·Toru SuzukiHiroshi Imai
Feb 11, 2012·Omics : a Journal of Integrative Biology·Sara L C EstevesEdgar F da Cruz e Silva
Jan 25, 2011·Molecular Human Reproduction·Margarida FardilhaEdgar da Cruz E Silva
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Sep 25, 2016·Journal of Molecular and Cellular Cardiology·David Y ChiangXander H T Wehrens

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