PMID: 15350191Sep 8, 2004Paper

Identification of the structural and functional determinants of the extracellular domain of the human follicle stimulating hormone receptor

The Journal of Endocrinology
Pallavi S KeneS D Mahale

Abstract

The extracellular domain (ECD) of the human follicle-stimulating hormone receptor (hFSHR) is believed to be the major determinant of hormone selectivity. Different discrete, discontinuous regions on the ECD of the hFSHR have been suggested to be crucial for hormone binding. However, the role of the ECD in signal transduction is not well understood. This study provides some insight into these aspects of the structure-function relationship of the ECD of hFSHR. Ten peptides were selected from the ECD on the basis of their ability to be surface oriented, synthesized by the solid-phase method using fluorenylmethyloxycarbonyl chemistry, purified and characterized. They were further studied for their ability to modulate both human follicle-stimulating hormone (hFSH)-FSHR binding and cAMP generation. Competitive inhibition studies showed that, of all the peptides studied, peptides 285-300 and 297-310 hFSHR were able to inhibit hFSH binding to FSHR. Both peptides function as weak competitive inhibitors of hFSH-FSHR binding. Peptides 285-300 hFSHR, 216-235 hFSHR, 184-195 hFSHR, 79-89 hFSHR and 15-31 hFSHR were observed to inhibit FSH-induced cAMP production. In summary, this study suggests that discrete, functional domains of the ECD hav...Continue Reading

Citations

Dec 3, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D PuJ Wu
Nov 13, 2009·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Ping YanJintao Li
Oct 15, 2009·Molecular and Cellular Endocrinology·Madhavi Dupakuntla, Smita D Mahale
Dec 1, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·P S KeneS D Mahale
May 11, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jenifer H AnklesariaSmita D Mahale
Nov 26, 2008·The Journal of Biological Chemistry·Gaurav Agrawal, Rajan R Dighe
Jun 20, 2018·Systems Biology in Reproductive Medicine·Vijaya GaneshSolomon Franklin Durairaj Paul
Sep 22, 2005·The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry·Peter FalkaiUNKNOWN WFSBP Task Force on Treatment Guidelines for Schizophrenia
Dec 1, 2020·The FEBS Journal·Antara A BanerjeeSmita D Mahale
Oct 17, 2020·Biochimica Et Biophysica Acta. Biomembranes·Kaushiki S PrabhudesaiSusan Idicula-Thomas
Feb 7, 2007·Journal of Reproductive Immunology·J D GhosalkarS D Mahale

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