PMID: 9537960Apr 16, 1998Paper

Identification of the structural similarity in the functionally related amidohydrolases acting on the cyclic amide ring

The Biochemical Journal
G J Kim, H S Kim

Abstract

The functionally related amidohydrolases, including D-hydantoinases, dihydropyrimidinases, allantoinases and dihydro-orotases, share a similar catalytic function of acting on the cyclic amide ring. We aligned 16 amidohydrolases by taking account of the conservative substitution and found a number of highly conserved regions and invariant amino acid residues. Analyses of the secondary structure and hydropathy profile of the enzymes revealed a significant degree of similarity in the conserved regions. Among the regions, the long stretched region I is of particular interest, because it is mainly composed of invariant amino acid residues, showing a similarity of 69% for the enzymes. A search of the protein data bank using the sequence of the conserved region I identified a number of proteins possessing a similar catalytic property, providing a clue that this region might be linked with the catalytic function. As a particular sequence, one aspartic acid and four histidine residues are found to be rigidly conserved in the functionally related amidohydrolases. In order to investigate the significance of the conserved residues, site-directed mutagenesis was carried out typically for the D-hydantoinase gene cloned from Bacillus stearoth...Continue Reading

Citations

Aug 28, 2013·Journal of Molecular Evolution·Matthieu BarbaBernard Labedan
Sep 26, 2001·Protein Expression and Purification·G J KimH S Kim
Oct 3, 2002·Biochemical and Biophysical Research Communications·Cheng-Yang Huang, Yuh-Shyong Yang
Apr 20, 2006·Molecular and Biochemical Parasitology·Sonia M Robles LopezBarbara H Zimmermann
Jun 2, 2005·Biotechnology Letters·Zihui Meng, Tomohiko Yamazaki
Jul 28, 2004·Archives of Microbiology·Dayna M BrichtaGerard A O'Donovan
Oct 8, 2005·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Sung-Hun NamHak-Sung Kim
Sep 17, 2013·Journal of Bacteriology·Anthony G DodgeLawrence P Wackett
Sep 11, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Cheng-Yang HuangYuh-Shyong Yang
Dec 19, 2001·The Journal of Biological Chemistry·Chee-Leong SoongSakayu Shimizu
Apr 23, 2008·Current Protocols in Protein Science·H Nagase
Sep 24, 2004·The Journal of Biological Chemistry·Anupama AhujaDavid R Evans
Dec 9, 2008·Journal of Bacteriology·Scott D PopeValley Stewart
May 2, 2000·Applied and Environmental Microbiology·G J KimH S Kim
Jul 1, 2017·The Protein Journal·Jacqueline VitaliMichael J Colaneri
May 7, 2019·Frontiers in Microbiology·Mikel Iradi-SerranoUnai Ugalde
Jul 9, 2011·The Protein Journal·Ya-Yeh HoCheng-Yang Huang
Sep 5, 2019·Biochemical and Biophysical Research Communications·Yen-Hua HuangCheng-Yang Huang
Feb 19, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·J AbendrothD Schomburg
Oct 3, 2003·Biochemical and Biophysical Research Communications·Jongchul YoonYoungsoo Kim
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Patrick J GainesNancy Wisnewski

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