Identification of the thymidylate synthase within the genome of white spot syndrome virus

The Journal of General Virology
Qin LiFeng Yang

Abstract

Thymidylate synthase (TS) (EC 2.1.1.45) is essential for the de novo synthesis of dTMP in prokaryotic and eukaryotic organisms. Within the white spot syndrome virus (WSSV) genome, an open reading frame (WSV067) that encodes a 289 amino acid polypeptide showed significant homology to all known TSs from species including mammals, plants, fungi, protozoa, bacteria and DNA viruses. In this study, WSV067 was expressed in Escherichia coli, and the purified recombinant protein showed TS activity in dUMP-folate-binding assays using ultraviolet difference spectroscopy. RT-PCR and Western blot analyses showed that WSV067 was a genuine and early gene. Phylogenetic analysis revealed that WSSV-TS was more closely related to the TSs of eukaryotes than to those from prokaryotes.

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Citations

Jun 21, 2006·BMC Bioinformatics·Hendrik MarksJust M Vlak
Jun 30, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Aldo A Arvizu-FloresRogerio R Sotelo-Mundo
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Jun 17, 2005·The Journal of General Virology·Hendrik MarksJust M Vlak
Nov 17, 2020·International Journal of Biological Macromolecules·Vipul PanchalDileep Vasudevan
May 4, 2021·Frontiers in Oncology·Juan SunWeiming Kang

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