Identification of the thyroid transcription factor-1 as a target for rat MST2 kinase.

The Journal of Biological Chemistry
L AurisicchioM Zannini

Abstract

Thyroid transcription factor-1 (TTF-1) is a homeodomain-containing transcription factor that is required for thyroid-specific expression of the thyroglobulin and thyroperoxidase genes as well as for lung-specific expression of the surfactant protein A, B, and C and the CC10 and the HNF-3 alpha genes. TTF-1 is a phosphoprotein, and the phosphorylation of TTF-1 has been studied already. However, the kinase(s) that could be responsible for this phosphorylation have not been known. In this paper we report the identification by in-gel kinase assay of a 56-kDa serine/threonine kinase that is able to phosphorylate TTF-1 in thyroid cells. The cloning of this kinase revealed that we had identified the rat homolog of the human MST2 kinase. The pathway in which human MST2 functions is not known; however, it does not appear to involve either mitogen-activated protein kinases such as Erk1 and Erk2 nor the stress-activated protein kinases such as JNK and p38. We show that the activity responsible for TTF-1 phosphorylation is constitutive in thyroid cells. Furthermore, we demonstrate that TTF-1 is phosphorylated in vivo by rMST2 at the same residues that had been identified previously as the major phosphorylation sites. Thus, TTF-1 represents...Continue Reading

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Citations

Apr 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chaeuk ChungDae-Sik Lim
Feb 27, 2001·The American Journal of Surgical Pathology·G PelosiG Viale
Nov 18, 2008·Experimental Cell Research·Tina Di PalmaMariastella Zannini
Jan 24, 2007·Physiological Reviews·Yutaka MaedaJeffrey A Whitsett
Jan 24, 2002·The Journal of Biological Chemistry·Kyung-Kwon Lee, Shin Yonehara
Nov 14, 1998·Biochimica Et Biophysica Acta·J A Whitsett, S W Glasser

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