Identification of the valid reference genes for quantitative RT-PCR in annual ryegrass (Lolium multiflorum) under salt stress

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Xia WangXinquan Zhang

Abstract

Annual ryegrass (Lolium multiflorum) is a cool-season annual grass cultivated worldwide for its high yield and quality. With the areas of saline soil increasing, investigation of the molecular mechanisms of annual ryegrass tolerance under salt stress has become a significant topic. qRT-PCR has been a predominant assay for determination of the gene expression, in which selecting a valid internal reference gene is a crucial step. The objective of present study was to evaluate and identify suitable reference genes for qRT-PCR in annual ryegrass under salt stress. The results calculated by RefFinder indicated that eEF1A(s) was the most stable reference gene in leaves, whereas EF1-a was the least stable; meanwhile, TBP-1 was the most optimal in roots and in all samples, and the eIF-5A shouldn't be utilized for normalization of the gene expression. eEF1A(s) is more suitable than TBP-1 as reference gene in leaves when verified with P5CS1 and Cyt-Cu/Zn SOD genes. We should choose optimal reference genes in specific tissues instead of the most stable one selected from different conditions and tissues.

References

Jul 31, 2004·BioTechniques·Keertan DhedaAlimuddin Zumla
Nov 19, 2005·BMC Molecular Biology·Pål A OlsvikIvar Hordvik
May 13, 2006·Biochemical and Biophysical Research Communications·Mukesh JainJitendra P Khurana
Dec 7, 2007·Plant Physiology and Biochemistry : PPB·Claudia O Silva-OrtegaJuan F Jiménez-Bremont
Jul 31, 2008·The Plant Cell·Michael K UdvardiWolf-Rüdiger Scheible
Apr 28, 2009·The Plant Cell·Ivo Rieu, Stephen J Powers
Apr 20, 2010·Biochemical and Biophysical Research Communications·Rohini GargMukesh Jain
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Dec 29, 2011·Molecular Genetics and Genomics : MGG·Xiaoyuan ChiShanlin Yu
Jan 27, 2012·Ecotoxicology and Environmental Safety·Huiying LiJinmin Fu
May 29, 2012·Journal of Microbiological Methods·C FlorindoM J Borrego
Jul 4, 2012·Gene·Laurence A M DeloffreAdelino V M Canario
Jul 3, 2013·Plant Physiology and Biochemistry : PPB·Lijuan WangPing Zhou
Sep 24, 2013·Molecular Biology Reports·Arash Kianianmomeni, Armin Hallmann
Feb 4, 2014·Microbiological Research·Nádia CastanheiraPaula Fareleira
Apr 15, 2014·Ecotoxicology and Environmental Safety·ZongBo QiuManMan Zhang
Sep 12, 2014·Plant Molecular Biology·Feng LinHan Zhao

❮ Previous
Next ❯

Citations

Jan 18, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qiuxu LiuXinquan Zhang
May 1, 2019·BMC Molecular Biology·Md Sabbir HossainMd Shahidul Islam

❮ Previous
Next ❯

Datasets Mentioned

BETA
EL664147.1
EL664154.1

Methods Mentioned

BETA
reverse transcription PCR
RNA-seq
salt treatment
PCR

Software Mentioned

Normfinder
geNorm
ΔCt
Primer Premier
BestKeeper
RefFinder

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.