PMID: 11926067Apr 3, 2002Paper

Identification of the YMN-1 antigen protein and biochemical analyses of protein components in the mitochondrial nucleoid fraction of the yeast Saccharomyces cerevisiae

Protoplasma
H SatoIsamu Miyakawa

Abstract

We analyzed the protein components contained in the mitochondrial nucleoid (mt-nucleoid) fraction of the yeast Saccharomyces cerevisiae. Immunoblotting with anti-Abf2p antibody demonstrated the association of Abf2p, a major mitochondrial DNA-binding protein, with the mt-nucleoids. In contrast, porin and cytochrome c oxidase subunit III (CoxIIIp) were not detected by immunoblotting in the mt-nucleoid fraction. The YMN-1 monoclonal antibody recognized a 48 kDa protein of the mt-nucleoid fraction. The N-terminal amino acid sequence of the protein and immunological evidence showed that the YMN-1 monoclonal antibody recognizes dihydrolipoyl transsuccinylase (KE2), which is one of the constituents of the alpha-ketoglutarate dehydrogenase complex (KGDC). alpha-Ketoglutarate dehydrogenase (KE1) and dihydrolipoyl dehydrogenase (E3), which are other subunits of KGDC, were also detected in the mt-nucleoid fraction. An enzyme assay of the mt-nucleoid fraction showed that cytochrome c oxidase and fumarase activity were barely detected in the fraction, but the specific activity of KGDC in the mt-nucleoid fraction was relatively high and was approximately 60% of the specific activity in the mitochondrial fraction. Three components of KGDC wer...Continue Reading

Citations

Jul 2, 2003·The Journal of General and Applied Microbiology·Isamu MiyakawaTomoko Nakaoka
Jan 24, 2009·The Journal of General and Applied Microbiology·Isamu MiyakawaYu Kadowaki
Feb 2, 2011·The Journal of General and Applied Microbiology·Isamu MiyakawaHiroshi Sato
Sep 15, 2011·The Journal of General and Applied Microbiology·Taihei FukudomiIsamu Miyakawa
May 9, 2012·Free Radical Biology & Medicine·Ariel R CardosoAlicia J Kowaltowski
Oct 13, 2006·Experimental Cell Research·Sharon KarnielyOphry Pines
Apr 25, 2006·FEMS Yeast Research·Jozef NosekIsamu Miyakawa
Jul 12, 2002·Yeast
Sep 30, 2003·Molecular & Cellular Proteomics : MCP·Daniel F BogenhagenRyuji Kobayashi
May 9, 2019·International Journal of Molecular Sciences·Arianna Barchiesi, Carlo Vascotto
Sep 25, 2004·Mass Spectrometry Reviews·Silas G Villas-BôasJens Nielsen
May 13, 2017·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Isamu Miyakawa
Feb 5, 2005·Science·Xin Jie ChenRonald A Butow

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